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目的:观察乙肝病毒(HBV)慢性感染者自体树突状细胞(DC)联合抗病毒治疗的临床效应.方法:采用自身前后对照试验,对41例HBV慢性感染者在试验前均应用IFN-α-2b治疗6 mo,无应答者停药6 mo后继续IFN-α-2b治疗6 mo,同时联合自体DC回输治疗,回输两次为一疗程.疗程结束后1mo检测受试者病毒学、血清学、生化学指标,在回输前后起始的检测指标均一致的基础上观察自身前后的变化.结果:体外培养扩增DC至第6天,HBsAg致敏形成特异性DC疫苗.DC回输后ALT和AST水平较回输前明显降低(t=2.976,P<0.01;t =2.049,P<0.05).HBV DNA应答率为53.7%,DNA拷贝数下降≥210g10.DC回输次数与病毒学应答反应没有相关性(r=0.048,P>0.05),HBV携带时间与病毒学应答反应亦没有相关性(r=0.137,P>0.05).结论:自体DC回输联合IFN-α-2b治疗HBV慢性感染者,生化学和病毒学单项应答反应均优于单纯IFN-α-2b治疗.
Objective: To observe the clinical effect of autologous dendritic cells (DCs) combined with antiviral therapy in patients with chronic hepatitis B virus infection.Methods: Before and after treatment, 41 patients with chronic HBV infection were treated with IFN-α -2b treatment for 6 months, non-responders discontinued 6 months after IFN-α-2b treatment 6 months, combined with autologous DC reinfusion treatment, twice for the return of a course of treatment .1mo after the end of the test subjects virology , Serological and biochemical indicators before and after reinfusion were detected on the basis of the same before and after changes in their own changes.Results: DC in vitro cultured to the first 6 days, the formation of specific DC vaccine sensitized by HBsAg.DC The levels of ALT and AST decreased significantly after transfusion compared with before transfusion (t = 2.976, P <0.01; t = 2.049, P <0.05) .HBV DNA response rate was 53.7% (R = 0.137, P> 0.05) .Conclusion: Autologous DC reinfusion combined with IFN-α (p <0.05) has no correlation with the virological response (r = -2b treatment of chronic HBV infection, biochemical and virological response to a single response were better than the simple IFN-α-2b treatment.