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采用三株分别定位于日本血吸虫表皮膜、肠道上皮和虫卵的单克隆抗体A6，SJ31／32和SE9作为捕获抗体建立检测三类循环抗原的斑点酶联免疫吸附实验（DOT－ELISA）方法，并利用该方法和普通ELISA分别观察了日本血吸虫感染家兔血清中三类循环抗原和IgG抗体的动态变化情况。结果表明：感染日本血吸虫家免在经砒喹酮治疗之后的第8周，睦相关抗原，可溶性虫卵抗原和肠相关抗原的转阴率分别为80％，70％和20％，而减后第18周全部转阴；IgG抗体一直维持较高水平。因此，MAA和SEA具有改好的疗效考核价值，GAA的疗效考核效果较差，IgG抗体不具有疗效考核作用。
Three monoclonal antibodies A6, SJ31 / 32 and SE9 located on the surface of Schistosoma japonicum epidermal membrane, intestinal epithelium and eggs were used as capture antibody to establish a dot-ELISA assay , And the dynamic changes of three kinds of circulating antigens and IgG antibodies in sera of rabbits infected with Schistosoma japonicum were observed by this method and ordinary ELISA respectively. The results showed that the rates of negative conversion rate of MSA, soluble egg antigen and intestinal-related antigen were 80%, 70% and 20% respectively in the 8th week after infection with Schistosoma japonicum, The first 18 weeks all negative; IgG antibodies have been maintained at high levels. Therefore, MAA and SEA have improved the value of efficacy assessment, GAA efficacy evaluation less effective, IgG antibody does not have the effect of assessment.