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目的 研究妊高征 (PIH)患者的血浆对体外培养的内皮细胞合成一氧化氮 (NO)及内皮型一氧化氮合成酶 (eNOSmRNA)表达的影响 ,探讨PIH患者血中NO水平的改变及改变的原因。 方法 在体外培养的脐静脉内皮细胞中分别加入PIH患者和正常晚孕妇女的血浆 ,2 4h后应用Northern杂交法比较加入不同血浆的内皮细胞eNOSmRNA表达的差异。 结果 加入PIH组血浆的内皮细胞eNOSmRNA的表达水平较加入正常晚孕组的内皮细胞的eNOSmRNA的表达水平增高 (P <0 .0 1)。 结论 eNOSmRNA表达增强可能是PIH时的一种代偿机制。
Objective To study the effect of plasma of PIH patients on the expression of nitric oxide (NO) and endothelial nitric oxide synthase (eNOS mRNA) in cultured endothelial cells in vitro and to explore the changes and changes of NO levels in blood of patients with PIH s reason. Methods The plasma of PIH patients and normal pregnant women were added to the cultured human umbilical vein endothelial cells respectively. The expression of eNOS mRNA in endothelial cells after adding different plasma was compared by Northern hybridization after 24 hours. Results The expression of eNOS mRNA in the endothelial cells of the PIH group was higher than that of the eNOS mRNA in the endothelial cells of the normal pregnancy group (P <0.01). Conclusions Enhanced eNOS mRNA expression may be a compensatory mechanism during PIH.