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目的:探讨正常人、Graves病(GD)和桥本甲状腺炎(HT)患者甲状腺细胞(TEC)凋亡率的差异,并观察凋亡相关蛋白的表达情况。方法:TUNEL法检测3种甲状腺组织的TEC凋亡率;免疫组化检测TEC凋亡蛋白Fas、FasL表达情况,RT-PCR检测FasmRNA表达的差异。结果:GD和HT患者TEC凋亡率显著高于正常对照(P<0.05),HT患者TEC凋亡率较GD患者也显著升高(P<0.05),FasL在正常、GD和HT甲状腺组织中存在连续性表达,但Fas在自身免疫性甲状腺疾病(AITD)患者的表达水平显著高于正常甲状腺组织,以HT的Fas表达水平最高(HT与正常对照相比,P<0.01;GD与正常对照比,P<0.05)。结论:凋亡可能在AITD的发病中起一定作用,Fas及其配基系统是与AITDTEC凋亡相关的蛋白。
Objective: To investigate the difference of apoptosis rate of thyroid cells (TEC) between normal controls, Graves’ disease (GD) and Hashimoto’s thyroiditis (HT) and to observe the expression of apoptosis related proteins. Methods: TUNEL method was used to detect the apoptosis rate of TEC in three thyroid tissues. The expression of Fas and FasL in TEC was detected by immunohistochemistry. The difference of Fas mRNA expression was detected by RT-PCR. Results: The TEC apoptosis rates in GD and HT patients were significantly higher than those in normal controls (P <0.05). The TEC apoptosis rates in HT patients were also significantly higher than those in GD patients (P <0.05). FasL was detected in normal, GD and HT thyroid tissues However, the expression of Fas in patients with autoimmune thyroid disease (AITD) was significantly higher than that in normal thyroid tissues (P <0.01). The expression of Fas in HT was higher than that in normal controls P <0.05). Conclusion: Apoptosis may play a role in the pathogenesis of AITD. Fas and its ligand system are proteins related to AITDTEC apoptosis.