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目的：研究白细胞介素－１α（ＩＬ－１α）诱导牛脑微血管内皮细胞（ＢＣＭＥＣ）释放血小板衍生性生长因子（ＰＤＧＦ），以及药物对由之引起的牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的拮抗作用。方法：体外培养ＢＣＭＥＣ和ＢＣＭＳＭＣ，结晶紫染色法测定细胞增殖。结果：ＩＬ－１α不能直接促进ＢＣＭＳＭＣ的增殖；但经ＩＬ－１α刺激的ＢＣＭＥＣ培养上清能显著地促进ＢＣＭＳＭＣ的增殖。这种增殖作用与ＩＬ－１α剂量呈正相关，并且可被抗ＰＤＧＦ抗体中和。欧芹素乙（Ｉｍｐ），异欧芹素乙（ｉｓｏ－Ｉｍｐ），６－（α，α－ｐｈｅｎｙ－ｌａｃｅｔｙｌｐｉｐｅｒａｚｉｎｙｌ）ｐｈｅｎｙｌ－５－ｍｅｔｈｙｌ－４，５－ｄｉｈｙｄｒｏ－３（２Ｈ）－ｐｙｒｉｄａｚｉｎｏｎｅ（ＰＭＤＰ）不影响ＩＬ－１α诱导ＢＣＭＥＣ释放ＰＤＧＦ，但对ＰＤＧＦ促ＢＣＭＳＭＣ增殖呈剂量依赖的拮抗。结论：ＩＬ－１α促进ＢＣＭＥＣ释放ＰＤＧＦ。ＩＬ－１α对ＢＣＭＳＭＣ增殖的促进作用需经ＰＤＧＦ等生长因子的介导。Ｉｍｐ，ｉｓｏ－Ｉｍｐ，ＰＭＤＰ拮抗ＰＤＧＦ引起的ＢＣＭＳＭＣ增殖。
AIM: To investigate the effects of interleukin-1α (IL-1α) on the release of platelet-derived growth factor (PDGF) from bovine brain microvascular endothelial cells (BCMECs) and on the proliferation of bovine microvascular smooth muscle cells (BCMSMCs) effect. Methods: BCMEC and BCMSMC were cultured in vitro and cell proliferation was measured by crystal violet staining. Results: IL-1α could not directly promote the proliferation of BCMSMC; however, the culture supernatant of BCMEC stimulated by IL-1α could significantly promote the proliferation of BCMSMC. This proliferative effect is positively correlated with IL-1α dose and can be neutralized by anti-PDGF antibodies. (Imp), iso-Imp, 6- (α, α-pheny-lacetylpiperazinyl) phenyl-5-methyl-4,5-dihydro-3 (2H) -pyridazinone ) Did not affect IL-1α-induced release of PDGF by BCMEC but dose-dependently antagonized the proliferation of PDGF-stimulated BCMSMC. Conclusion: IL-1α promotes BCMEC release of PDGF. The promoting effect of IL-1α on the proliferation of BCMSMC needs to be mediated by growth factors such as PDGF. Imp, iso-Imp, PMDP antagonized PDGF-induced BCMSMC proliferation.