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目的 构建实用型低密度石英谐振式生物传感器 (quartz oscillation biosensor,QOBS)阵列检测系统。方法 首先利用精细微加工法制作单个 QOBS,在此基础上先后构建了粘接式以及夹具式 2× 5型 QOBS阵列 ;并自行开发研制自激式振荡电路、PESA V2 .0版频率记录分析软件 ;将它们与计算机联用以构建 QOBS阵列检测系统。结果 成功地制造出了在气、液相中稳定振荡的单个 QOBS,构建的粘接式及夹具式 QOBS阵列各有其优缺点 ;自激式振荡电路有非常强的振荡能力 ,在液相中起振非常容易 ,其频率稳定度也可达实用要求 ;PESAV2 .0版频率记录分析软件具有自行设置各初始参数 ,实时记录频率变化、自动绘制频率变化趋势图及分析结果等多项功能。结论 成功地构建出 QOBS阵列检测系统 ,搭建起一个生物检测的技术平台 ,为临床上病原性微生物的检测以及多种疾病的早期诊断提供一种新的方法。
Objective To construct a practical low-density quartz oscillation biosensor (QOBS) array detection system. Methods Firstly, a single micro-fabrication method was used to fabricate a single QOBS. On the basis of that, a bonded QXBS array with a 2 × 5 fixture was built successively. Self-excited oscillation circuit, PESA V2.0 frequency record analysis software ; Combine them with computers to build a QOBS array detection system. As a result, a single QOBS with stable oscillation in gas and liquid phase was successfully fabricated, and the advantages and disadvantages of the bonded and jigged QOBS arrays were established. The self-excited oscillation circuit has a very strong oscillating ability in the liquid phase Start-up is very easy, its frequency stability can reach the practical requirements; PESAV2 .0 version of the frequency analysis software records have set their own initial parameters, real-time recording frequency changes, automatically plot the trend of frequency and analysis results and many other features. Conclusion The QOBS array detection system was successfully constructed and a bio-detection technology platform was established to provide a new method for the detection of pathogenic microorganisms and the early diagnosis of various diseases.