论文部分内容阅读
观察白血病抑制因子 (LIF)受体gp190亚基完整的细胞内区和gp190胞内区C末端片段(190CT)对人白血病系HL 6 0表达CD14、CD15的影响 ,进一步了解LIF引发白血病细胞增殖抑制和分化的关系 .用基因重组技术将LIF另一亚基gp130的细胞内区换成gp190的细胞内区 ,用PCR技术扩增gp190细胞内区C末端的一个多肽的编码序列 ,构成嵌合体受体基因 130 /190及 190CT片段 ,并分别在HL 6 0细胞表达 .用免疫组化和流式细胞术检测分析在LIF的诱导下 ,HL 6 0细胞表达CD14、CD15的水平 .转染pcDNA130 /190的HL 6 0细胞 ,CD15表达量明显增高 ;转染pcDNA190CT的细胞 ,CD15的表达量降低 ;但 2组细胞的CD14表达量均较低且水平接近 .LIF可能诱导HL 6 0细胞向粒细胞而不向单核细胞分化 ,该效应是由gp190亚基细胞内区介导的 ,而gp190C末端片段可干扰LIFα受体介导的信号传导效应 .
To observe the effects of intact intracellular domain of gp190 subunit of leukemia inhibitory factor (LIF) receptor and C-terminal fragment of intracellular domain gp190 (190CT) on the expression of CD14 and CD15 in human leukemia HL 6 0 cells, and to further understand the inhibitory effects of LIF on leukemia cell proliferation And differentiation.Using gene recombination technology, the intracellular region of gp130, another subunit of LIF, was changed into the intracellular region of gp190, and the coding sequence of a polypeptide at C-terminal of gp190 cell region was amplified by PCR to form a chimera 130/190 and 190CT fragments were expressed in HL 60 cells respectively.The expression of CD14 and CD15 was detected by immunohistochemistry and flow cytometry in HL60 cells under the induction of LIF.The transfected pcDNA130 / 190 HL60 cells, CD15 expression was significantly increased; transfected pcDNA190CT cells, CD15 expression decreased, but the two groups of cells were lower and the level of CD14 expression.LIF may induce HL 60 cells to granulocytes Without differentiation into monocytes, this effect is mediated by the intracellular domain of gp190 subunit, whereas the gp190C terminal fragment interferes with LIFa receptor-mediated signaling.