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目的 研究中药解毒通络保肾胶囊对糖尿病 (DM)大鼠肾脏病变的保护作用及其机制。方法 将实验动物分为正常对照组、DM对照组、阳性对照组和实验组。检测各组第 12周的血糖、尿素氮、血肌酐、尿白蛋白排泄率 (UAER)、血管紧张素Ⅱ (AngⅡ )含量及肾脏肥大指标的变化 ,应用RT PCR检测肾皮质结缔组织生长因子 (CTGF)mRNA表达 ,免疫组化检测肾内纤维连接蛋白 (FN)和Ⅳ型胶原 (ColⅣ )蛋白表达水平。结果 解毒通络保肾胶囊较DM对照组血糖显著降低 (P <0 0 1) ,UAER明显减少 (P <0 0 5 ) ,尿素氮和血肌酐水平下降 ,肾脏肥大指标改善 (P <0 0 1)。RT PCR结果表明DM大鼠肾皮质CTGFmRNA表达升高 ,肾小球FN、ColⅣ沉积增多 ,解毒通络保肾胶囊治疗组CTGFmRNA表达水平和Fn和ColⅣ蛋白表达均低于DM对照组 (P <0 0 1)。结论 解毒通络保肾胶囊对DM肾脏病变有保护作用 ,其机制可能是减少肾组织中AngⅡ含量 ,下调CTGF的表达 ,减少细胞外基质的沉积
Objective To study the protective effect of Jiedu Tongluobaoshen capsule on renal lesions in diabetic rats and its mechanism. Methods The experimental animals were divided into normal control group, DM control group, positive control group and experimental group. Blood glucose, urea nitrogen, serum creatinine, urinary albumin excretion rate (UAER), angiotensin II (AngII) content, and renal hypertrophy were measured at week 12 in each group. Renal cortical connective tissue growth factor was measured by RT PCR ( CTGF) mRNA expression was detected by immunohistochemistry to detect the expression levels of fibronectin (FN) and type IV collagen (ColIV) proteins in the kidney. Results Compared with DM control group, Jiedu Tongluo Baoshen Capsules significantly reduced blood glucose (P <0 01), UAER decreased significantly (P <0 05), blood urea nitrogen and serum creatinine levels decreased, and renal hypertrophy index improved (P <0. 1). The result of RT PCR showed that the expression of CTGF mRNA in renal cortex of diabetic rats increased, and the deposition of glomerular FN and Col IV increased. The expression of CTGF mRNA and Fn and Col IV protein in Jiedu Tongluobao capsule group were lower than those in DM control group (P<0). 0 1). Conclusion Jiedu Tongluobaoshen Capsule has protective effect on renal lesions of DM. The mechanism may be to decrease the content of AngII in renal tissue, down-regulate the expression of CTGF, and reduce the deposition of extracellular matrix.