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目的:观察比较正常人与冠心病患者外周血平滑肌前体细胞(SPCs)数量的差异,以及冠心病患者在经皮冠脉介入治疗(PCI)前后外周血中SPCs数量的变化。方法:将研究对象分为3组:稳定型心绞痛(SAP)组、不稳定型心绞痛(UAP)及正常对照组(NC)。SPCs以CD14和CD105双阳性确定,利用流式细胞仪双色分析技术筛选各组在PCI术前、术后即刻、术后72 h SPCs占外周血有核细胞的百分比。结果:PCI术前,外周血中SPCs数量在SAP组为(0.20±0.13)%、UAP组为(0.28±0.18)%,均明显高于NC组[(0.12±0.10)%](均P<0.01),UAP组外周血中SPCs数量明显高于SAP组(P<0.01)。UAP组外周血中SPCs的数量在PC I术后72 h较术前明显增加[(0.34±0.25)%vs.(0.28±0.18)%,P<0.01]。结论:外周血中SPCs数量的变化及PC I对外周血SPCs数量的影响可能与冠心病的临床类型有关。
Objective: To observe the difference of the number of peripheral blood smooth muscle cells (SPCs) between normal subjects and patients with coronary heart disease (CHD) and the changes of the numbers of SPCs in peripheral blood before and after percutaneous coronary intervention (PCI). Methods: The subjects were divided into three groups: SAP group, UAP group and normal control group (NC). SPCs were double positive by CD14 and CD105. The percentage of peripheral blood mononuclear cells in each group before and after PCI was screened by flow cytometry two-color analysis at 72 h after PCI. Results: The number of SPCs in peripheral blood before and after PCI was (0.20 ± 0.13)% in SAP group and (0.28 ± 0.18)% in UAP group [(0.12 ± 0.10)%] (P < 0.01). The number of SPCs in UAP group was significantly higher than that in SAP group (P <0.01). The number of SPCs in peripheral blood of UAP group was significantly increased at 72 h after PCI (P <0.01) compared with that before operation (0.34 ± 0.25)% vs (0.28 ± 0.18)%. CONCLUSION: The changes of the number of SPCs in peripheral blood and the influence of PC I on the number of SPCs in peripheral blood may be related to the clinical type of coronary heart disease.