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目的:近年研究发现白细胞介素-18(Interleukin18,IL-18)在冠心病(CAD)的发生、发展及粥样斑块破裂的过程中起重要作用。本研究探讨IL-18基因-607C/A和-137G/C单核苷酸多态与中国北方汉族人群心肌梗死(MI)的关系。方法:采用序列特异性引物聚合酶链反应对432例对照组和468例MI患者进行检测,分析IL-18基因-607C/A和-137G/C单核苷酸多态的基因型和等位基因分布情况。结果:IL-18基因-607C/A单核苷酸多态3种基因型(CC型,CA型和AA型)在对照组分布频率分别为20.8%,50.9%和28.2%,在MI组分别为36.3%,44.9%和18.8%,IL-18基因-607C/A多态和中国北方汉族人群MI的发生显著相关(P<0.05)。IL-18基因-137G/C单核苷酸多态三种基因型(GG型,GC型和CC型)在对照组分布频率分别为71.3%,26.8%和1.9%,在MI组分别为75.2%,23.9%和0.9%,IL-18基因-137G/C单核苷酸多态与中国北方汉族人群MI无相关性(P=0.133)。两组间的基因型分布皆符合Hardy-Weinberg平衡定律。Logistic回归校正性别、年龄、体质量指数、吸烟、高血压病、高脂血症、糖尿病等CAD易患因素后,IL-18基因-607C/A多态仍是MI发病的独立的危险因素(P<0.05)。IL-18基因-607C/A和-137G/C单核苷酸多态组成的CG单体型与和MI的危险性呈正相关,而AC单体型与MI的危险性呈负相关。结论:IL-18基因-607C/A多态与中国北方汉族人群中与MI的发生独立相关。
OBJECTIVE: In recent years, Interleukin 18 (IL-18) has been found to play an important role in the development of coronary heart disease (CAD) and the development of atherosclerotic plaque rupture. This study was to investigate the relationship between -607C / A and -137G / C single nucleotide polymorphisms of IL-18 gene and myocardial infarction (MI) in Han population in northern China. Methods: 432 control subjects and 468 MI patients were detected by sequence-specific primer polymerase chain reaction. The genotypes and alleles of IL-18 gene -607C / A and -137G / C single nucleotide polymorphisms Gene distribution. Results: The frequencies of -607C / A single nucleotide polymorphism (CC, CA and AA) of IL-18 gene in control group were 20.8%, 50.9% and 28.2% Was 36.3%, 44.9% and 18.8%, respectively. The polymorphism of IL-18 gene -607C / A was significantly associated with MI in northern Chinese Han population (P <0.05). IL-18 gene -137G / C SNP genotypes (GG type, GC type and CC type) in the control group were 71.3%, 26.8% and 1.9%, respectively, in the MI group were 75.2 %, 23.9% and 0.9%, respectively. There was no correlation between -137G / C single nucleotide polymorphism of IL-18 gene and MI in northern Chinese Han population (P = 0.133). Genotype distribution between the two groups are in line with the Hardy-Weinberg equilibrium law. Logistic regression corrected the predisposing factors of CAD such as sex, age, body mass index, smoking, hypertension, hyperlipidemia and diabetes mellitus, IL-18 gene-607C / A polymorphism is still an independent risk factor for MI P <0.05). The CG haplotypes of IL-18 gene -607C / A and -137G / C SNPs were positively correlated with the risk of MI, while the AC haplotypes were negatively correlated with the risk of MI. CONCLUSION: The -607C / A polymorphism of IL-18 gene is independently associated with the occurrence of MI in northern Chinese Han population.