论文部分内容阅读
【目的】探讨不同神经生长因子对神经干细胞(neural stemcells,NSCs)分化特性的影响。【方法】自10～14周人胚胎脑分离培养海马神经干细胞,分别用表皮生长因子(human epidermal growth factor,EGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF),或单独用bFGF扩增神经干细胞,取第3代神经干细胞球,用含小剂量bFGF(0.5 ng)的无血清基础培养基诱导分化,体外诱导分化7 d后应用免疫荧光方法检测β_Ⅲ微管蛋白(β_ⅢTubulin,Tuj1)和神经胶质纤维酸性蛋白(glial fibriliary acidic protein,GFAP)表达情况。【结果】不同的生长因子培养的神经干细胞分化特性很不相同,EGF+bFGF培养的NSCs既可分化为神经元,又可分化为星形胶质细胞,其分化比率分别为38.8%和57.3%;bFGF培养的NSCs主要分化为神经元,比率达81.2%,未检测星形胶质细胞。【结论】不同神经生长因子扩增的神经干细胞分化特性很不相同,本实验为神经干细胞分化的研究提供了良好的实验平台。
【Objective】 To investigate the effect of different nerve growth factors on differentiation characteristics of neural stem cells (NSCs). 【Methods】 Hippocampal neural stem cells were isolated and cultured in human embryonic brain from 10 to 14 weeks, respectively. Human epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) The bFGF-derived neural stem cells were harvested, and the third generation of NSCs were harvested and induced to differentiate with low-dose bFGF (0.5 ng) serum-free basal medium. After induced for 7 days in vitro, immunofluorescence was used to detect β_III tubulin Tuj1) and glial fibrillary acidic protein (GFAP). 【Results】 The differentiation characteristics of NSCs differentiated by different growth factors were very different. The NSCs cultured with EGF + bFGF could differentiate into neurons and differentiate into astrocytes with differentiation rates of 38.8% and 57.3% The NSCs cultured in bFGF mainly differentiated into neurons with the ratio of 81.2%. Astrocytes were not detected. 【Conclusion】 Differentiation of NSCs differentiated by different nerve growth factors is very different. This experiment provides a good experimental platform for neural stem cell differentiation research.