论文部分内容阅读
目的:分析结直肠组织多不饱和脂肪酸(PUFA)的成分水平,以及与相关肿瘤因子的相关性。方法:新鲜冷冻的肿瘤组织与正常组织取自2010-12-2011-03在中国人民解放军总医院普通外科行结直肠癌切除术的82例患者。应用气相色谱毛细管层析法检测组织中PUFA成分水平。并行酶联免疫技术测定组织中肿瘤相关细胞因子。结果:肿瘤组织中总ω-6PUFA、亚油酸(LA C18:2)显著低于正常组织,P=0.00。总ω-3PUFA和花生三烯酸(DGLA C20:3)在肿瘤组织中显著增高,P=0.00。花生四烯酸(AA C20:4)/亚油酸(LA)及AA/总ω-6PUFA在肿瘤组织中明显增高,P=0.00。同一患者肿瘤组织中前列腺素E2(PGE2)、血小板衍生因子(PDGF)、环氧化酶(COX-2)及血管内皮生长因子(VEGF)相比正常组织偏高。其中,PDGF、COX-2及VEGF差异有统计学意义(P=0.01,P=0.00)。在研究对象中,LA与COX-2呈负相关(r=-0.324 4,P<0.05),AA/总ω-6PUFA、AA/LA(r=0.308 3和0.300 1,P<0.05)。结论:结直肠癌及正常组织多不饱和脂肪酸的代谢存在差异,肿瘤组织中消耗ω-6PUFA并产生代谢产物,有助于局部炎症微环境的产生,从而可能促进了肿瘤的发生及发展。
OBJECTIVE: To analyze the compositional level of polyunsaturated fatty acids (PUFA) in colorectal tissues and its correlation with related tumorous factors. Methods: Fresh frozen tumor tissues and normal tissues were obtained from 82 patients who underwent colorectal resection in the General Surgery General Hospital of Chinese People’s Liberation Army from December 2010 to November 2011. Gas chromatography-capillary chromatography was used to detect the level of PUFA in the tissue. Parallel enzyme-linked immunosorbent assay for tumor-associated cytokines in tissues. Results: Total omega-6 PUFAs and linoleic acid (LA C18: 2) in tumor tissue were significantly lower than those in normal tissues (P = 0.00). Total omega-3 PUFA and arachidonic acid (DGLA C20: 3) were significantly higher in tumor tissue with P = 0.00. Arachidonic acid (AA C20: 4) / linoleic acid (LA) and AA / total omega-6 PUFAs were significantly increased in tumor tissue, P = 0.00. PGE2, PDGF, COX-2 and VEGF in the same patient were higher than normal tissues. Among them, the difference of PDGF, COX-2 and VEGF was statistically significant (P = 0.01, P = 0.00). LA and COX-2 were negatively correlated (r = -0.324 4, P <0.05), AA / total omega-6 PUFA, AA / LA (r = 0.308 3 and 0.300 1, P <0.05) in the study subjects. CONCLUSIONS: There are differences in the metabolism of polyunsaturated fatty acids in colorectal cancer and normal tissues. Overexpression of ω-6PUFA in tumor tissues and production of metabolites contribute to the production of local inflammatory microenvironment, which may promote the occurrence and development of tumors.