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1植物名称浙皖粗筒苣苔(Briggsia chienii Chun)。2材料类别种子及无菌苗叶片。3培养条件MS为基本培养基。诱导种子发芽培养基:(1)MS+6-BA 1.0 mg·L~(-1)(单位下同)+NAA 0.5;诱导叶片分化培养基:(2)MS+6-BA 2.0+NAA 0.5;增殖继代培养基:同(1);壮苗培养基:(3)MS+6-BA0.1+NAA 0.05;生根培养基:(4)1/2MS+0.1%活性炭。以上培养基均含30 g·L~(-1)蔗糖和7.0 g·L~(-1)琼脂,pH 5.8。培养温度为(25±2)℃;光照强度约为30
1 plant name Zhejiang and Anhui borage moss (Briggsia chienii Chun). 2 material types of seeds and sterile seedling leaves. 3 culture conditions MS as the basic medium. Induced seed germination medium: (1) MS + 6-BA 1.0 mg · L -1 (unit same below) + NAA 0.5; induced leaf differentiation medium: (2) MS + 6-BA 2.0 + NAA 0.5 (3) MS + 6-BA0.1 + NAA 0.05; rooting medium: (4) 1/2 MS + 0.1% activated charcoal. The above medium contained 30 g · L -1 sucrose and 7.0 g · L -1 agar at pH 5.8. Incubation temperature (25 ± 2) ℃; light intensity of about 30