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利用SSR分子标记对唐山、天津的96个红小豆品种(按品种地理来源划分为14个品种群)进行遗传多样性分析,结果表明,197对引物中共有89对的扩增谱带清晰稳定,共扩增出285条带,多态性条带百分率(PPB)达100%,经Popgene32软件分析,遵化品种资源的遗传多样性水平最高[PPB=85.39%,I(信息指数)=0.567],其次为玉田和迁安的品种资源,而在天津红小豆资源中,除静海县(排位第5)外,遗传多样性水平相对较小,其中宁河的遗传多样性水平最低(PPB=22.47%,I=0.1575)。聚类分析表明,96个品种可分为三个组群,唐山红小豆组群中包含了天津静海小豆;天津红小豆中的大港品种资源单独为一组群;其他天津红小豆为第二大组群。本研究可为地方品种的保存及杂交组配提供参考。
The genetic diversity of 96 adzuki bean cultivars (divided into 14 cultivars by geographical source) of Tangshan and Tianjin was analyzed by SSR markers. The results showed that 89 pairs of amplification bands of 197 pairs of primers were stable and stable, A total of 285 bands were amplified and the percentage of polymorphic bands (PPB) was 100%. The genetic diversity of Zunhua cultivars was the highest (PPB = 85.39%, I (informative) = 0.567) , Followed by Yutian and Qian’an cultivars, while in Tianjin adzuki beans, except for Jinghai (rank no. 5), the genetic diversity was relatively small, of which Ninghe had the lowest genetic diversity (PPB = 22.47%, I = 0.1575). Cluster analysis showed that 96 cultivars could be divided into three groups. The population of Tangshan red adzuki beans included Tianjin Jinghai adzuki beans; the population of Dadonghai adzuki beans among Tianjin adzuki beans was a single group; the other Tianjin red adzuki beans was the second largest group. This study can provide reference for the preservation of local varieties and cross-breeding.