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目的对5株不同年份分离的猪链球菌进行病原学特征分析,以评估其对公共卫生的危害。方法对分离菌株进行毒力相关基因检测、脉冲场凝胶电泳分析(PFGE)。结果所有菌株的相关毒力因子cps2A、map、sly和ef均为阳性。酶切图谱显示2006、2007年菌株图谱完全一致,2010年分离菌与2006、2007年分离株存在差异。结论分离菌株有相当强的致病性,从人和猪分离到菌株酶切图谱完全一致,显示为统一来源或者同一克隆群;不同地域分离的菌株酶切图谱相似度较低。
Objective To analyze the etiological characteristics of Streptococcus suis isolates from five different years to evaluate their harm to public health. Methods The isolates were tested for virulence related genes and analyzed by pulse field gel electrophoresis (PFGE). Results The virulence factors cps2A, map, sly and ef of all strains were positive. The enzyme digestion map showed that the strains were completely identical in 2006 and 2007, and there was a difference between isolates and strains isolated in 2006 and 2007. CONCLUSION: The isolates have quite strong pathogenicity. The digested isolates from human and pig are completely identical to each other and show a uniform source or the same clonal group. The isolates from different regions have lower similarity.