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利用保绿法和萝卜子叶增重法从7年生甜樱桃[Cerasus pseudocerasus(Lindl.)G.Don]根际土壤中,筛选具有促生作用的植物根际促生细菌YT-3(PGPR),以发酵好的鸡粪(DCM)为吸附载体制成甜樱桃专用的PGPR生物肥料(YMF),对比研究了YMF、普通生物肥(NMF)和DCM对樱桃根系和根际土壤生物学特征的影响.结果表明:YMF显著增加了根际土壤中细菌数量和微生物总量,真菌数量明显减少,但对放线菌数量影响差异不显著.YMF处理根系活力分别比NMF、DCM和CK提高了22.49%、13.25%和15.33%.PGPR生物肥料对樱桃根系生长和构建影响显著,YMF处理0～40 cm土壤剖面中根系重量尤其是毛细根重量显著增加.同NMF处理相比,YMF处理根际土壤的pH降低8.61%,阳离子代换能力显著提高.此外,YMF处理显著增加了根际土壤中养分离子的有效性,速效磷和有效钾含量分别增加17.21%和9.56%,但碱解氮含量差异不显著.因此,PGPR生物肥的施用在一定程度上改善了根际土壤的生态环境,提高了根际土壤中养分离子的有效性和养分保持能力,提高了根系活力,促进了表层土壤中(主要为0～40 cm)根系尤其是毛细根的生长.
Plant growth promoting rhizobacteria YT-3 (PGPR) were screened from the roots of 7-year-old sweet cherry [Cerasus pseudocerasus (Lindl.) G.Don] The effect of YMF, common biological fertilizer (NMF) and DCM on the soil biological characteristics of cherry root and rhizosphere was studied by using fermented chicken manure (DCM) as adsorption carrier to make PGPR biological fertilizer (YMF) The results showed that YMF significantly increased the amount of bacteria and total amount of microorganisms in rhizosphere soils, and the number of fungi decreased obviously, but the effect on YMF was not significant.The root activity of YMF was increased by 22.49% , 13.25% and 15.33%, respectively.PGPR biofertilizer had a significant effect on root growth and architecture of cherry, and the root weight, especially capillary root weight in 0-40 cm soil profile increased significantly in YMF treatment.Compared with NMF treatment, YMF treatment of rhizosphere soil In addition, YMF treatment significantly increased the availability of nutrient ions in the rhizosphere soil, and available phosphorus and available potassium increased by 17.21% and 9.56%, respectively, but the differences in the content of alkaline nitrogen were not significant Significantly, therefore, PGPR bio-fat It can improve the rhizosphere soil ecological environment to a certain extent, improve the availability of nutrient ions and nutrient retention ability in rhizosphere soils, increase root activity and promote root system (mainly 0-40 cm) in surface soil, especially It is the growth of capillary root.