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为探讨肥胖对致死性肺炎全身性免疫反应的影响,将高脂诱导的肥胖小鼠分为Ⅰ、Ⅱ组,非肥胖小鼠分为Ⅲ、Ⅳ组,Ⅰ、Ⅲ组滴鼻40 L大肠杆菌菌液(4×10~(10) CFU),Ⅱ、Ⅳ组滴鼻40 L生理盐水,检测感染前(第0小时)及感染后第2、6、12、24、48、72、96小时各组小鼠血液白细胞数、血清细胞因子质量浓度及免疫器官指数。结果显示,高脂饲喂8周后,肥胖组小鼠体重,血液WBC、GRA、MID数量,血清TNF-α、IL-6、RETN质量浓度均显著高于非肥胖组(P<0.05),脾脏和胸腺的质量及指数显著低于非肥胖组(P<0.05)。感染后,Ⅰ、Ⅲ组血液WBC、GRA、LYM、MID数量在感染后第2小时略降低,后逐渐升高后又降低,在第48小时达到最小值;与Ⅲ组比较,Ⅰ组的WBC数量在第0~6小时,GRA数量在第0~12小时,MID数量在第0、6小时均显著升高(P<0.05),LYM数量在感染后第48小时显著降低(P<0.05);Ⅰ、Ⅲ组的TNF-α、IL-6、IL-10、RETN质量浓度迅速升高,且维持在较高水平至第48小时;Ⅰ组的TNF-α质量浓度在第0~6、24小时,IL-6质量浓度在第0~6、96小时,IL-10质量浓度在第2~12、48~96小时及RETN质量浓度在第0~2、24~48小时显著高于Ⅲ组(P<0.05);Ⅰ组的体重在感染后第2~96小时,胸腺质量在第6~96小时及胸腺指数在第12、72~96小时显著低于Ⅱ组(P<0.05),脾质量和脾指数在感染后第2~96小时显著高于Ⅱ组(P<0.05),Ⅰ组的体重在第0~96小时,脾质量在第2~12、48~96小时,脾指数在第2、12、48小时及胸腺质量在第24小时显著高于Ⅲ组(P<0.05)。上述结果表明,肥胖能通过影响机体脾脏及胸腺的生长发育、白细胞及细胞因子含量,进而影响肺部感染引起的炎症反应强度和免疫调节能力,导致致死性肺炎肥胖小鼠的死亡率增高。
In order to investigate the effect of obesity on the systemic immune response to lethal pneumonia, obese mice induced by high fat diet were divided into groups Ⅰ and Ⅱ, and non-obese mice were divided into groups Ⅲ and Ⅳ. Groups of 40 L E.coli (4 × 10 ~ (10) CFU), and the rats in group Ⅱ and Ⅳ were given intranasal 40 L of saline to detect the infection before infection (0 hour) and the 2nd, 6th, 12th, 24th, 48th, 72th and 96th hour after infection The number of white blood cells, serum cytokines and immune organ index of mice in each group. The results showed that body weight, blood WBC, GRA, MID, serum TNF-α, IL-6 and RETN in obese group were significantly higher than those in non-obese group (P <0.05) The quality and index of spleen and thymus were significantly lower than those of non-obese group (P <0.05). After infection, the numbers of WBC, GRA, LYM and MID in blood of Group Ⅰ and Ⅲ decreased slightly at the 2nd hour after infection, then gradually increased and then decreased and reached the minimum at the 48th hour. Compared with the Ⅲ group, the WBC, The number of MID increased significantly at 0h and 6h (P <0.05) and the number of LYM decreased significantly (P <0.05) at the 48th hour after infection, The concentrations of TNF-α, IL-6, IL-10 and RETN in group Ⅰ and group Ⅲ increased rapidly and maintained at a high level up to the 48th hour. The concentration of TNF- At 24 hours, the concentration of IL-6 was significantly higher than that of 0 ~ 2, 94 ~ 48 hours at 0 ~ 6,96 hours after IL-10 concentration at the 2nd ~ 12th ~ 48th ~ 96th hour and RETN concentration at 0 ~ (P <0.05). The body weight of group Ⅰ was significantly lower than that of group Ⅱ (P <0.05) on the 2nd to 96th hour after infection, the thymus mass was on the 6th to 96th hour and the thymus index was on the 12th, 72th to 96th hour Spleen mass and spleen index were significantly higher in group 2 to 96 hours after infection than those in group Ⅱ (P <0.05). The body weight of group Ⅰ was between 0 and 96 hours and spleen mass was between days 2 to 12 and 48 to 96 hours after spleen index At the 2nd, 12th and 48th hour, the thymus mass was significantly higher in the 24th hour than in the third group (P <0.05). The above results show that obesity can affect mortality and mortality of fatal pneumonia obese mice by affecting the growth and development of the spleen and thymus, leukocytes and cytokines, thereby affecting the inflammatory response intensity and immunomodulatory capacity caused by pulmonary infection.