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为了解抗青蒿琥酯恶性疟原虫对本芴醇、蒿甲醚、双氢青蒿素是否存在交叉抗性 ,本芴醇与青蒿琥酯伍用是否有增效作用 ,运用 Rieckmann体外微量法测定。结果上述 3种药物对青蒿琥酯敏感株恶性疟原虫的 ID5 0 分别为 72 .3、14.6及 13.4nm ol/ L ;对抗性株的 ID5 0 依次为 6 6 .8、80 .5及 72 .6 nmol/ L。蒿甲醚、双氢青蒿琥酯对抗性株的 ID5 0 分别较敏感株高出 4.5及 4.4倍 ,本芴醇对敏感株的 ID5 0 与抗性株相似。在本芴醇与青蒿琥酯伍用中 ,两者对抗性株的 ID5 0 分别为 44 .8及 39.6 nmol/ L ,为单用组的 1/ 1.5 4(44 .7/ 6 6 .8)和 1/ 2 .2 (39.6 / 85 .1)。结果提示抗青蒿琥酯恶性疟原虫对本芴醇无交叉抗性 ,青蒿琥酯与本芴醇伍用在体外测定中具有一定增效作用 ;抗青蒿琥酯恶性疟原虫对蒿甲醚、双氢青蒿素有明显的交叉抗性
In order to find out whether there is a synergistic effect of the combination of lumefantrine and artesunate on the anti-Artesunate falciparum cross-resistance to lumefantrine, artemether and dihydroartemisinin, the Rieckmann in vitro micro-assay Determination. Results The ID50 values of the three drugs against artesunate-sensitive P. falciparum were 72.3, 14.6 and 13.4 nmol / L, respectively. The ID50s of the resistant strains were 66.8, 80.5 and 72 .6 nmol / L Artemether and dihydroartemisinin-resistant ID50s were 4.5 and 4.4 times higher than those of the susceptible strains, respectively. The ID50 of benflumetol against susceptible strains was similar to that of the resistant strains. In the combination of lumefantrine and artesunate, the ID50s of the two antagonistic strains were 44.8 and 39.6 nmol / L, respectively, which were 1 / 1.54 (44.7 / 66.8 ) And 1/2 .2 (39.6 / 85 .1). The results suggest that anti-artesunate falciparum non-cross-resistance to lumeflumeol, artesunate and bendroflunexin Wu in vitro assay has a synergistic effect; artesunate Plasmodium falciparum artesunate , Dihydroartemisinin obvious cross-resistance