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为探讨肿瘤转移与细胞表面的糖结构的关系，对小鼠肝癌细胞的高、低淋巴道转移株Ｈｃａ－Ｆ和Ｈｃａ－Ｐ进行了蛋白质电泳及经蛋白质印迹术后的５种凝集素（ＣｏｎＡ、ＷＧＡ、ＵＥＡ、ＳＢＡ、ＰＮＡ）结合糖蛋白谱的对比分析．结果表明：高、低转移两株细胞的ＳＤＳ－ＰＡＧＥ谱基本相同；ＣｏｎＡ特异结合糖蛋白共有５种（～７２，８０～９０，～１０４，～１５０，～２００ｋＤ）；其中较明显的差异为～７２ｋＤＣｏｎＡ特异结合糖蛋白，它在Ｈｃａ－Ｐ细胞的表达明显高于Ｈｃａ－Ｆ细胞．ＷＧＡ特异结合糖蛋白１种（～１５０ｋＤ），在Ｈｃａ－Ｐ细胞的表达略高于Ｈｃａ－Ｆ细胞．此外，实验发现两种性质未明的蛋白质（～７９，～１３０ｋＤ），后者在Ｈｃａ－Ｐ细胞的含量明显高于Ｈｃａ－Ｐ细胞．结果提示Ｈｃａ－Ｆ和Ｈｃａ－Ｐ细胞不同的转移表型可能与其糖蛋白的表达有一定的关联．
To investigate the relationship between tumor metastasis and glycostructures on the cell surface, protein electrophoresis was performed on the Hca-F and Hca-P high and low lymph node metastases of mouse hepatoma cells and five lectins after Western blotting were performed (ConA). , WGA, UEA, SBA, PNA) Contrast analysis of glycoprotein profiles. The results showed that the SDS-PAGE profiles of high and low metastatic cells were basically the same; ConA specifically binds to glycoproteins in 5 species (~72, 80-90, -104, -150, -200 kD); the more obvious difference is ~72kDConA specifically binds to glycoprotein and its expression in Hca-P cells is significantly higher than that of Hca-F cells. WGA specifically binds to one glycoprotein (~150kD) and is slightly higher in Hca-P cells than Hca-F cells. In addition, two unspecified proteins (~79, ~130kD) were found in the experiment, and the latter was significantly higher in Hca-P cells than Hca-P cells. The results suggest that different metastatic phenotypes of Hca-F and Hca-P cells may be associated with their glycoprotein expression.