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本文研究心房特殊颗粒 (ASG)中的钙释放是否能促进颗粒内容的分泌。冷冻超薄切片技术和磷钨酸—乙醇 (EPTA)特殊染色技术制备大鼠心房切片。X射线显微定量分析采用Hall连续谱法 ,用以测量在肾上腺素处理后 15、30、6 0和 12 0min时ASG中钙含量的变化。用形态计量法的数密度作为判断ASG分泌状态的指标。结果显示心房特殊颗粒在生理状态下钙浓度高达 80mmol 公斤·干重。在肾上腺素注射后ASG的数密度逐渐下降 ,并于注射后 6 0min时下降至最低 ;ASG中的钙含量亦逐渐减低 ,但于注射后 30min即达最低 ,由此可见ASG钙含量的下降出现早于颗粒数的下降。推想肾上腺素处理引起的钙释放可能成为一种信号 ,启动心房肽的分泌 ,因此ASG在心房心肌细胞中可能具有细胞内钙库机能。本文同时讨论了Hall连续谱法近年来在生物试样定量分析中的应用和发展
This article investigates whether calcium release in atrial specific granules (ASG) can promote the secretion of particulate content. Frozen ultrathin sections and EPTA special staining techniques were used to prepare rat atrial sections. X-ray microanalysis was performed using Hall continuum spectroscopy to measure changes in calcium content in ASG at 15, 30, 60 and 120 min after epinephrine treatment. The number density of morphometry was used as an index to judge the secretion status of ASG. The results showed that the atrium special particles in the physiological state of calcium concentration up to 80mmol kg · dry weight. The number density of ASG decreased gradually after injection of epinephrine and decreased to the lowest at 60 min after injection. The content of calcium in ASG also decreased gradually but reached the lowest level at 30 min after injection, indicating that the decrease of ASG calcium content appeared As early as the decline in the number of particles. It is hypothesized that calcium release from adrenergic treatment may serve as a signal that initiates the secretion of atrial natriuretic peptide and therefore ASG may have intracellular calcium reservoir function in atrial cardiomyocytes. This paper also discusses the application and development of Hall continuum spectrometry in the quantitative analysis of biological samples in recent years