论文部分内容阅读
目的观察雷米普利抑制肾素-血管紧张素系统(RAS)阻断后对糖尿病(DM)大鼠胰岛再生衍生因子3α(Reg3α)表达的影响。方法 SD大鼠10只采用链脲菌素40mg/kg腹腔注射建立DM模型:B组5只为模型对照(用生理盐水灌胃10周);C组5只采用雷米普利50mg·kg-1·d-1灌胃10周。另取5只大鼠腹腔注射等量柠檬酸缓冲液作为空白组(A组,用生理盐水灌胃10周)。基因芯片检测大鼠胰岛相关基因表达;实时定量PCR和Western blot验证相关基因mRNA和蛋白表达。结果 B组Reg3α表达较A组上调7.9倍,C组较B组Reg3α表达上调7.1倍。C组胰岛染色面积和胰岛形态结构较B组增加和改善。结论雷米普利抑制胰岛局部RAS系统,促进DM大鼠的胰岛再生;其作用可能部分通过影响Reg3α基因的表达实现。
Objective To investigate the effect of ramipril on the expression of Regulatory factor 3α (Reg3α) in the islet of diabetic rats after inhibition of renin - angiotensin system (RAS). Methods 10 SD rats were injected with streptozotocin 40mg / kg intraperitoneally to establish DM model: 5 rats in group B were given model control (10 weeks with saline); 5 rats in group C were treated with ramipril 50 mg · kg- 1 · d-1 for 10 weeks. Another 5 rats were intraperitoneally injected with the same amount of citrate buffer as a blank group (group A, normal saline for 10 weeks). Gene microarray was used to detect islet related gene expression in rat. Real-time quantitative PCR and Western blot were used to verify the mRNA and protein expression of related genes. Results The expression of Reg3α in group B was up-regulated by 7.9-fold compared with that in group A, while the expression of Reg3α in group C was 7.1-fold more than that in group B. The islet staining area and islet morphology in group C were increased and improved compared with group B. Conclusion Ramipril inhibits the islet local RAS system and promotes the islet regeneration in DM rats. The effect of ramipril may be partly through the influence of Reg3α gene expression.