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目的探讨脐血IgE、IL-4、IL-12水平对婴儿变态反应性疾病发生的预测价值及三者之间的相互变化关系。方法应用美国IMX型化学发光检测仪,采用免疫荧光方法测定129例新生儿脐血IgE水平;采用双抗体夹心ELISA法测定新生儿脐血IL-4、IL-12水平,并进行3 a随访。根据是否出现变态反应情况分为变态反应组和正常组。结果变态反应组脐血IgE、IL-4水平均较正常组明显增高(Pa<0.01),而IL-12水平较后者明显降低(P<0.01)。变态反应性疾病患儿脐血的3种介质相关分析显示IL-4与IgE呈显著正相关(r=0.878,P<0.01);IL-12与IgE呈显著负相关(r=-0.643,P<0.01);IL-12与IL-4呈显著负相关(r=-0.711,P<0.01)。结论脐血IgE、IL-2、IL-5水平检查可预测婴儿变态反应性疾病的发生,从而发现高危儿,对儿童避免变态反应性疾病的发生和指导医疗保健有重要意义。
Objective To investigate the predictive value of cord blood IgE, IL-4 and IL-12 levels in infants with allergic diseases and their relationship with each other. Methods IMX chemiluminescence detector was used in the study. IgE level in cord blood of 129 neonates was measured by immunofluorescence method. IL-4 and IL-12 levels were measured by double antibody sandwich ELISA and followed up for 3 years. According to whether the occurrence of allergic reaction is divided into allergy group and normal group. Results The levels of IgE and IL-4 in cord blood of patients with allergic reaction were significantly higher than those of normal group (P <0.01), while the levels of IL-12 were significantly decreased (P <0.01). Correlation analysis showed that there was a significant positive correlation between IL-4 and IgE (r = 0.878, P <0.01), IL-12 and IgE were negatively correlated (r = -0.643, P <0.01). There was a significant negative correlation between IL-12 and IL-4 (r = -0.711, P <0.01). Conclusion The examination of IgE, IL-2 and IL-5 levels in cord blood can predict the occurrence of allergic diseases in infancy, and thus find that high-risk infants are of great significance to children in preventing the occurrence of allergic diseases and guiding medical care.