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目的探讨丙型病毒性肝炎抗病毒治疗无应答及复发患者白细胞介素-28B基因的多态性。方法丙型病毒性肝炎患者73例,均采用聚乙二醇干扰素联合利巴韦林标准方案治疗,其中抗病毒治疗复发者26例为复发组,无应答者22例为无应答组,获得持续病毒学应答(sustained virological response,SVR)者25例为SVR组。采用基因测序法检测3组白细胞介素-28B基因多态性位点rs12979860和rs8099917,比较3组各位点基因型分布情况。结果无应答组和复发组白细胞介素-28B基因rs12979860CT基因型比例(77.3%,57.7%)和rs8099917TG基因型比例(77.3%,57.7%)明显高于SVR组(8.0%,8.0%)(P<0.05),rs12979860CC基因型比例(18.2%,42.3%)和rs8099917TT基因型比例(18.2%,42.3%)明显低于SVR组(92.0%,92.0%)(P<0.05),无应答组与复发组比较差异均无统计学意义(P>0.05);73例中rs12979860CT杂合基因及rs8099917TG杂合基因者均为34例,其中复发者和无应答者共32例(94.15%),明显高于SVR患者2例(5.85%)(P<0.05)。结论丙型病毒性肝炎患者抗病毒治疗无应答及复发与其白细胞介素-28B基因多态性密切相关。
Objective To investigate the polymorphism of interleukin-28B gene in patients with unresponsive and recurrent hepatitis C virus antiviral therapy. Methods 73 cases of hepatitis C patients were treated with peginterferon combined with ribavirin standard regimen, of which 26 cases relapsed after antiviral therapy were relapse group, 22 cases were non-responders without response group, access to The sustained virological response (SVR) were 25 cases of SVR group. Genotypes were used to detect rs12979860 and rs8099917 polymorphisms of interleukin-28B gene in three groups. Genotype distribution of each group was compared. Results The proportion of rs12979860CT genotype (77.3%, 57.7%) and rs8099917TG genotype (77.3%, 57.7%) in non-responder group and relapse group were significantly higher than those in SVR group (8.0%, 8.0%) 0.05). The genotypes of rs12979860CC (18.2%, 42.3%) and rs8099917TT (18.2%, 42.3%) were significantly lower than those of SVR (92.0%, 92.0%) (P <0.05) (P> 0.05). There were 34 cases of rs12979860CT heterozygous gene and rs8099917TG heterozygous gene in 73 cases (94.15%), which were significantly higher than that of 32 cases (94.15%) Two cases of SVR patients (5.85%) (P <0.05). Conclusion No response and relapse of antiviral therapy in patients with viral hepatitis C are closely related to the polymorphism of interleukin - 28B gene.