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目的用磁共振成像方法研究中耳免疫反应诱导的动物内淋巴积水。方法将9只豚鼠分为2组,中耳免疫组(4只)和磷酸盐缓冲液(Phosphate buffered saline,PBS)对照组(5只)。先用KLH加福氏完全佐剂行四肢趾蹼免疫,2周后将KLH投放至中耳进行局部免疫。另外5只豚鼠中耳放置PBS作为对照组。用gadolinium增强的磁共振成像观察内耳淋巴液的动态变化,用耳蜗电图评估听功能改变。结果在KLH中耳免疫的动物中,2只发生内淋巴积水,3只血迷路屏障通透性增加,2只听力损失大于10dB。结论磁共振成像可以显示KLH中耳免疫反应的耳蜗改变,内淋巴积水和血迷路屏障通透性的增加提示存在着一个“漏的迷路”,将有可能为内耳疾病的诊断提供更加精确的依据。
Objective To study the middle ear immunoreaction - induced endolymphatic hydrops in animals using magnetic resonance imaging. Methods Nine guinea pigs were divided into two groups, the middle ear immunized group (4) and the phosphate buffered saline (PBS) control group (5). First KLH plus Freunds complete adjuvant line limbs toe webbed immunization, 2 weeks after the release of KLH to the middle ear for local immunization. Another five guinea pigs were placed in the middle ear as a control group. Dynamic changes of the inner ear lymph were observed with gadolinium-enhanced magnetic resonance imaging and the auditory function was evaluated using the cochlear electrogram. RESULTS: In the animals immunized with KLH middle ear, 2 developed endolymphatic hydrops and increased the permeability of the 3 blood labyrinth barrier with 2 hearing loss greater than 10 dB. Conclusions Magnetic resonance imaging can show cochlear changes in the immunoreactivity of the middle ear of KLH. The increased endolymphatic hydrops and permeability of the blood labyrinth barrier suggests a “lost labyrinth” that may provide a more accurate in accordance with.