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采用双抗体夹心ELISA法,对69例结直肠癌(CRC)患者手术前后血清可溶性白细胞介素-2受体(sIL-2R)和肿瘤坏死因子(TNF)水平进行了动态观察,结果表明:①结直肠癌患者血清sIL-2R和TNF水平均明显高于正常对照(P<0.01);②结直肠Dukes分期C期和D期的sIL-2R和TNF水平均明显高于A期和B期(P<0.05);③结直肠癌患者手术后1个月;血清sIL-2R和TNF水平均显著降低(P<0.05),④手术后1个月,行根治性手术者血清sIL-2R和TNF水平均明显降低(P<0.01),但仍比正常高;行姑息性手术者血清sIL-2R和TNF水平有所降低,但无显著差别(P>0.05),二组间有显著差异(P<0.05);⑤血清sIL-2R和TNF之间有显著的正相关性(r=0.374,P<0.01),二者并且对结直肠癌的临床分期、手术疗效评估及预后判断均有参考价值。
The serum soluble interleukin-2 receptor (sIL-2R) and tumor necrosis factor (TNF) levels in 69 patients with colorectal cancer (CRC) before and after surgery were observed by double-antibody sandwich ELISA. The results showed that: 1 The serum levels of sIL-2R and TNF in colorectal cancer patients were significantly higher than those in normal controls (P<0.01). 2 The levels of sIL-2R and TNF in the colorectal Dukes stage C and D were significantly higher than those in stages A and B ( P<0.05);3One month after operation in patients with colorectal cancer, serum sIL-2R and TNF levels were significantly decreased (P<0.05).4Serial sIL-2R and TNF were detected in patients who underwent radical surgery 1 month after surgery. The level of blood was significantly lower (P<0.01), but it was still higher than normal; serum sIL-2R and TNF levels were reduced in patients undergoing palliative surgery, but there was no significant difference (P>0.05). There was a significant difference between the two groups (P <0.05);5There was a significant positive correlation between serum sIL-2R and TNF (r=0.374, P<0.01), both of which have reference value for the clinical staging, surgical evaluation and prognosis of colorectal cancer. .