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目的了解本地区秋冬季婴幼儿病毒性腹泻标本中轮状病毒、诺如病毒、星状病毒和肠道腺病毒流行现况,为病毒性腹泻的诊治提供依据。方法收集某医院2009年9月-2010年3月间就诊入院的急性腹泻患儿粪便252份,采用ELISA法检测轮状病毒(Human Rotavirus,HRV)、人杯状病毒(Human Caliciviruses,HuCV)、肠道腺病毒(Enteric Adenovirus,EAdV)和星状病毒(Astrovirus,AstV)四种病毒抗原,并进行统计分析。结果 252例标本总的病毒检出率为73.02%(184/252)。单纯HRV阳性率为53.97%(136/252);单纯NV阳性率为10.32%(26/252),其中合并HRV感染3例;单纯AstV阳性率为4.76%(12/252),其中合并HRV感染2例;单纯EAdV阳性率为3.97%(10/252)。结论轮状病毒仍然是本地区引起婴幼儿秋冬季腹泻的主要病原,人杯状病毒、肠道腺病毒和星状病毒被认为是引起腹泻的常见病原,有病毒混合感染现象。
Objective To understand the prevalence of rotavirus, norovirus, astrovirus and enteric adenovirus in infants with viral diarrhea in autumn and winter in this area and provide basis for the diagnosis and treatment of viral diarrhea. Methods A total of 252 excrement samples collected from a hospital with acute diarrhea admitted to hospital from September 2009 to March 2010 were collected. Human rotavirus (HRV), human caliciviruses (HuCV) Enteric Adenovirus (EAdV) and Astrovirus (AstV) four virus antigens, and statistical analysis. Results The total positive rate of 252 samples was 73.02% (184/252). The positive rate of pure HRV was 53.97% (136/252). The positive rate of simple NV was 10.32% (26/252), of which 3 cases were complicated with HRV infection. The positive rate of pure AstV was 4.76% (12/252) 2 cases; simple EAdV positive rate was 3.97% (10/252). Conclusions Rotavirus is still the main cause of diarrhea in infants and young children in autumn and winter in this area. Human calicivirus, enteric adenovirus and astrovirus are considered as common pathogens causing diarrhea with mixed virus infection.