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目的探讨血管内皮生长因子(VEGF)及其受体VEGFR-2(KDR)和细菌L型感染在卵巢肿瘤中的表达及临床相关性研究。方法应用免疫组化、原位杂交和革兰染色等方法检测了120例卵巢肿瘤中的VEGF、KDR蛋白及mRNA的表达,以及细菌L型的检出率。并对97例卵巢乳头状癌和23例卵巢乳头状瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果VEGF、KDR蛋白及mRNA阳性表达率恶性肿瘤明显高于良性肿瘤(P<0.005)。细菌L型检出阳性率与卵巢良、恶性肿瘤差异无显著性(P>0.5)。VEGF、KDR蛋白及mRNA阳性表达以及细菌L型检出阳性率与卵巢乳头状癌的临床分期、病理分级和腹腔淋巴结有转移、腹水差异有显著性(P<0.001～0.05)。细菌L型阳性患者中VEGF、KDR阳性明显高于L型阴性患者中阳性表达,2组差异具有非常显著性(P<0.0001)。结论VEGF、KDR蛋白及mRNA在卵巢肿瘤中有不同程度的异常表达,与卵巢癌的临床分期、病理分级和浸润、转移呈正相关,L型感染极有可能成为诱发肿瘤因素之一,它们可能有协同致瘤作用。研究L型感染与卵巢肿瘤的关系,具有重要的临床应用价值。
Objective To investigate the expression and clinical correlation of vascular endothelial growth factor (VEGF), its receptor VEGFR-2 (KDR) and bacterial L-type infection in ovarian tumors. Methods The expression of VEGF, KDR protein and mRNA in 120 ovarian tumors and the detection rate of bacterial L type were detected by immunohistochemistry, in situ hybridization and Gram stain. And 97 cases of papillary carcinoma of ovary and 23 cases of ovarian papilloma were compared between the main clinical data and pathological grading parameters, using χ2 test for statistical analysis. Results The positive rates of VEGF, KDR protein and mRNA in malignant tumors were significantly higher than those in benign tumors (P <0.005). The positive rate of bacterial L-type detection was not significantly different from that of benign and malignant ovarian tumors (P> 0.5). The positive expression rates of VEGF, KDR protein and mRNA, as well as the positive rate of bacterial L-type detection and the clinical stage, histological grade and lymph node metastasis of ascites were significantly different (P <0.001 ~ 0.05). The positive expression of VEGF and KDR in patients with positive L-forms of bacteria was significantly higher than that in patients with negative L-forms, and the difference between the two groups was very significant (P <0.0001). Conclusions The expressions of VEGF and KDR protein and mRNA are abnormally expressed in ovarian cancer with different degrees, which are positively correlated with the clinical stage, histological grade and infiltration and metastasis of ovarian cancer. L-type infection is highly likely to be one of the factors inducing tumor. They may have Collaborative tumorigenic effect. Study of L-type infection and ovarian cancer has important clinical value.