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目的研究血卟啉单甲醚介导的光、声动力联合杀伤C6细胞的效应并对其机制进行初步分析。方法实验分为对照组、声动力组、单纯激光光照组、光动力组和光动力+声动力组。在光、声动力联合组中,首先采用强度为0.5 W/cm2、频率为1 MHz的超声照射,之后应用不同能量密度(40、80、120、160、200和240 J/cm2)的激光进行照射。应用MTT方法检测细胞死亡率,采用流式细胞仪检测细胞凋亡,荧光分析仪检测细胞内活性氧水平的变化。结果超声剂量固定为0.5 W/cm2,频率1 MHz条件下,当激光能量密度小于200 J/cm2时,声、光动力联合组C6细胞的杀伤率明显高于任何单一模式。当激光能量密度为80 J/cm2时,声、光动力联合组所诱导的C6细胞凋亡率最高。声、光动力联合可明显增加细胞内活性氧的产量。结论光、声动力联合能明显增加C6细胞杀伤率。
Objective To study the effect of hematoporphyrin-monomethyl ether-mediated combination of light and acousto-power on killing C6 cells and to analyze its mechanism. Methods The experiment was divided into control group, acousto-dynamic group, laser-only group, photodynamic group and photodynamic + acoustodynamic group. In the combined optical and acousto-dynamic group, first ultrasound with an intensity of 0.5 W / cm2 and a frequency of 1 MHz was used, followed by laser with different energy densities (40, 80, 120, 160, 200 and 240 J / cm2) Irradiation. The cell death rate was detected by MTT assay. Apoptosis was detected by flow cytometry. Fluorescence analyzer was used to detect changes of intracellular reactive oxygen species. Results When the ultrasonic dose was fixed at 0.5 W / cm2 and the frequency was 1 MHz, the killing rate of C6 cells in acousto-photodynamic therapy group was obviously higher than that in any single mode when the laser energy density was less than 200 J / cm2. When laser energy density was 80 J / cm2, the apoptosis rate of C6 cells induced by the combination of sound and photodynamic therapy was the highest. Sound, photodynamic combination can significantly increase intracellular reactive oxygen species production. Conclusions Light and sound power combination can significantly increase the killing rate of C6 cells.