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目的 :探讨谷氨酰胺对大鼠小肠结构和吸收氨基酸功能的营养保护作用。方法SD大鼠分为 3组 :A组为正常对照组 ;B组为氟尿嘧啶组 ,通过胃管连续两天给予大鼠氟尿嘧啶 12 5mg/ (kg·d) ;C组为氟尿嘧啶 +谷氨酰胺组 ,自给氟尿嘧啶的当日 ,饮水中加入 3%的谷氨酰胺。观察 3组大鼠给药后第 1天至第 7天大鼠每日饮食量的变化、给药前和给药后第 8天分别称大鼠体重、给药后第 8天用图象分析仪进行小肠形态学变化分析、用在体门静脉取血法观察小肠对色氨酸吸收能力的变化、测定动脉血谷氨酰胺浓度。结果 :给药后第 1天～第 7天B组饮食量明显低于正常 ,尤其是第 2～ 4天最为严重 ,而C组从第 6天起 ,饮食量与对照组相比无显著差异 ;B组体重下降明显 ,而C组体重下降明显减轻 ;B组小肠结构有明显损伤、小肠对色氨酸的吸收明显下降、动脉血谷氨酰胺浓度明显降低 ,C组小肠结构和吸收色氨酸的功能均有明显改善 ,动脉血谷氨酰胺浓度有明显升高。结论 :谷氨酰胺对氟尿嘧啶诱导的小肠结构损伤和吸收功能的障碍有明显的营养保护作用
Objective: To investigate the nutrition protective effect of glutamine on intestinal structure and amino acid absorption in rats. Methods SD rats were divided into three groups: group A was normal control group; group B was fluorouracil group, which was administered with 250 mg / (kg · d) of fluorouracil for two consecutive days through gastric tube; group C was fluorouracil plus glutamine group , The day of self-administered fluorouracil, add 3% glutamine in the drinking water. The daily dietary changes of rats in the three groups were observed on the first day to the seventh day after administration, and the body weights of the rats before administration and on the 8th day after the administration were respectively measured by image analysis on the 8th day Instrument for morphological changes of small intestine analysis, the use of the portal vein to observe the small intestine tryptophan absorption capacity changes, determination of arterial blood glutamine concentration. Results: From the 1st to the 7th day after administration, the diet of B group was significantly lower than normal, especially the most severe on the 2nd to 4th days. However, there was no significant difference in the diet between the 6th day and the C group ; Body weight decreased significantly in group B, and body weight decreased significantly in group C. The intestinal structure of group B was significantly damaged, the intestinal absorption of tryptophan decreased significantly, the concentration of arterial glutamine decreased significantly, Acid function were significantly improved, arterial blood glutamine concentration was significantly higher. CONCLUSION: Glutamine has a significant nutritional protective effect on fluorouracil-induced impairment of intestinal structure and absorption