Granulocyte colony-stimulating factor increases extracellular glutamic acid uptake and suppresses fr

• 作 者: Shengzhe Zheng Lei Song Lei Lu
• 机 构: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2011年2期
• 关键词: amyotrophic lateral sclerosis glutamic acid granulocyte colony-stimulating facto

Excitatory amino acid toxicity and free radical damage play important roles in amyotrophic lateral sclerosis.Granulocyte colony-stimulating factor (G-CSF) protects nerve cells exposed to high-concentrations of glutamic acid, suggesting positive effects in the treatment of amyotrophic lateral sclerosis.The present study induced in vitro motor neuron injury using glutamic acid excitotoxicity, and the biochemical effects of G-CSF on glutamic acid concentration were determined.In addition, the effects of G-CSF on superoxide dismutase, glutathione peroxidase activity in motor neurons, and malondialdehyde and nitric oxide contents were analyzed.Immunohistochemistry was performed to measure neuronal survival.Results revealed that G-CSF significantly suppressed free radical activity, inhibited excitotoxicity, and reduced apoptosis and loss of motor neurons in the anterior h of the spinal cord.