论文部分内容阅读
目的探讨心房颤动时犬心房内膜面一氧化氮(N0)浓度,血浆纤溶酶原激活剂抑制物-1(PAI-1)含量的变化。方法应用埋藏式高频心脏起搏器以400次/分的频率起搏心房6周,建立犬房颤动物模型。利用ISO-NOP3005NO敏感电极测定心内膜NO浓度,酶联免疫吸附双抗体夹心法测定血浆PAI-1含量。结果房颤组左房心内膜NO浓度明显低于窦性心律组,血浆PAI-1含量明显高于窦性心律组。结论房颤致左房心内膜NO浓度降低、血浆PAI-1含量升高,可能导致左房血栓的形成。
Objective To investigate the changes of nitric oxide (N0) and plasminogen activator inhibitor-1 (PAI-1) in atrial fibrillation in dogs. Methods Atrial fibrillation model was established by implanting a high frequency cardiac pacemaker at a rate of 400 beats per minute for 6 weeks. Endocardial NO concentration was measured by ISO-NOP3005NO sensitive electrode, and plasma PAI-1 content was measured by enzyme-linked immunosorbent assay (ELISA). Results The atrial endocardial NO concentration in atrial fibrillation group was significantly lower than that in sinus rhythm group. The content of PAI-1 in plasma was significantly higher than that in sinus rhythm group. Conclusion Atrial fibrillation results in decreased NO concentration in the left atrium and increased plasma PAI-1 levels, which may lead to the formation of left atrial thrombus.