宫颈癌脆性组氨酸三联基因的表达与血浆中的甲基化状态

• 作 者: 任晨春 苗绪红 杨斌 赵磊 孙蕊 宋文芹
• 机 构: 天津市中心妇产科医院,南开大学生命科学院,南开大学生命科学院,南开大学生命科学院,南开大学生命科学院,南开大学生命科学院 300052南开大学生命科学院
• 出 处: 中华医学遗传学杂志
• 发表日期: 2006年05期
• 关键词: FHIT基因 血浆 甲基化 免疫组化 宫颈癌

目的探讨宫颈癌患者血浆中脆性组氨酸三联(fragile histidine triad,FHIT)基因5′端CpG岛甲基化状态以及该基因在宫颈癌组织中的表达情况。方法用甲基化特异性聚合酶链反应(methylation specific polymerase chainreaction,MS-PCR)对151例患者的血浆进行FHIT基因甲基化状态的研究,并用免疫组织化学方法检测FHIT蛋白的表达情况。结果 31 .13 %的患者血浆FHIT基因发生甲基化;59 .60 %宫颈癌组织FHIT蛋白表达减弱或缺失,其中47 .78 %存在血浆FHIT基因的甲基化。结论血浆中FHIT基因甲基化是引起宫颈癌FHIT蛋白表达减弱的重要因素,利用它可以对宫颈癌进行无创伤诊断和预后的评估。

Objective To investigate the methylation status of 5 ’end CpG island of fragile histidine triad (FHIT) gene and its expression in cervical cancer in patients with cervical cancer. Methods Methylation-specific polymerase chain reaction (MS-PCR) was used to study the methylation status of FHIT gene in plasma of 151 patients. The expression of FHIT protein was detected by immunohistochemistry. Results The plasma FHIT gene methylation occurred in 31.13% of the patients. The expression of FHIT protein in 60% of cervical cancer tissues was decreased or absent, of which 47.78% had methylation of plasma FHIT gene. Conclusion The methylation of FHIT gene in plasma is an important factor that leads to the decrease of FHIT protein expression in cervical cancer. It can be used to evaluate the noninvasive diagnosis and prognosis of cervical cancer.