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目的为了寻找全氟异丁烯(PFIB)致急性肺损伤的防治药物。方法用气相色谱测定PFIB染毒浓度及氯磷定与PFIB的化学反应转化率。小鼠吸入PFIB染毒24h后,观察小鼠用氯磷定治疗后肺重量系数的变化,测定小鼠肺灌洗液(BALF)中总蛋白含量。结果用氯磷定预防给药组动物的肺重量系数及BALF中总蛋白含量显著降低,同时,中毒动物的存活率也明显提高。用气相色谱测定的氯磷定与PFIB的化学反应转化率为64·8%,说明PFIB比较容易与肟基离子结合。以上结果说明,氯磷定可降低PFIB的毒性,氯磷定对PFIB致急性肺损伤的防治作用很可能与它的化学亲核反应有关。结论氯磷定抑制了PFIB中毒小鼠肺组织的蛋白漏出,降低了中毒动物的肺重量系数和提高了存活率。氯磷定对PFIB中毒具有一定预防抗毒效果。
Objective To find out the prevention and treatment of acute lung injury caused by perfluoroisobutylene (PFIB). Methods The concentration of PFIB and the conversion of chlorophosphorus to PFIB were determined by gas chromatography. Mice inhaled PFIB exposure 24h after the mice treated with chlorophosphorus treatment of lung weight coefficient changes in the determination of total protein in lung lavage fluid (BALF). Results The lung weight coefficient and the total protein in BALF were significantly decreased in the group treated with chlorpromazine, and the survival rate of the poisoned animals was also significantly increased. The chemical conversion of chlorophosphorus determined by gas chromatography with PFIB was 64.8%, indicating that PFIB was easier to combine with oxime ion. The above results show that, chlorpromazine can reduce the toxicity of PFIB, chlorpromazine on the prevention and treatment of PFIB-induced acute lung injury is likely to be related to its chemical nucleophilic reactions. CONCLUSION Chlorfluidin inhibits protein leakage in the lungs of PFIB-poisoned mice, decreases the lung weight coefficient and increases the survival rate of the poisoned animals. Chloride and phosphorus poisoning PFIB poisoning has some anti-drug effect.