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目的:观察苯巴比妥钠、γ-氨基丁酸(GABA)联合二巯基丙磺酸钠(Na-DMPS)对毒鼠强急性中毒大鼠的治疗作用。方法:将30只同种SD大鼠随机分为3组:正常对照组、单纯染毒组、联合用药治疗组;每组10只,雌雄各半。通过大鼠急性毒性实验观察染毒后即刻给予苯巴比妥钠、γ-氨基丁酸联合二巯基丙磺酸钠的解毒作用,于死亡立即或2h后取血测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酸(AST)、NSE、S-100β蛋白,并观察各组脑组织、肝脏的病理学改变。结果:联合用药治疗组能有效缓解中毒大鼠症状,延长抽搐潜伏期及惊厥潜伏期(P<0.05);联合用药治疗组大鼠血清ALT、AST、NSE、S-100β蛋白与单纯染毒组比较显著降低(P<0.05),与正常对照组比较差异无统计学意义(P>0.05);联合用药治疗组大鼠脑组织、肝脏的病理改变较单纯染毒组大鼠的病理改变轻。结论:苯巴比妥钠、γ-氨基丁酸联合二巯基丙磺酸钠对急性毒鼠强中毒大鼠有治疗作用。
Objective: To observe the therapeutic effect of phenobarbital sodium, γ-aminobutyric acid (GABA) combined with sodium dimercaptopropane sulfonate (Na-DMPS) on rats with acute poisoning. Methods: Thirty SD rats of the same type were randomly divided into 3 groups: normal control group, simple exposure group and combined treatment group; 10 in each group, male and female were divided into two groups. Through acute toxicity test in rats, the detoxification effect of phenobarbital sodium and γ-aminobutyric acid combined with sodium dimercaptopropane sulfonate was observed immediately after the exposure. Serum alanine aminotransferase (ALT ), Aspartate aminotransferase (AST), NSE, S-100βprotein, and observed the pathological changes of brain tissue and liver in each group. Results: The combination treatment group can effectively relieve the symptoms of poisoning rats, prolong the latent period of seizure and the latent period of convulsion (P <0.05). The serum ALT, AST, NSE and S-100β in the combined treatment group were significantly higher than those in the purely poisoned group (P <0.05). There was no significant difference compared with the normal control group (P> 0.05). The pathological changes of the brain tissue and liver in the combined treatment group were lighter than those in the purely poisoned group. CONCLUSION: Phenobarbital sodium and γ-aminobutyric acid combined with sodium dimercaptopropanesulfonate have a therapeutic effect on rats with acute tetramine poisoning.