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采用不同年龄组豚鼠随机分甲、乙两组.甲组按年龄3、6、12个月龄分为甲_1、甲_2、甲_3组(统称自然衰老组);而乙组仅取3个月龄的豚鼠(下称人工促衰老组),于眼球后注射促衰老制剂持续32天.各组行测听(ABR)后处死,测其心、脑中的丙二醛(Malondialdehyed,MDA)及超氧化物歧化酶(Superoxide dismutase,SOD)活力并于扫描电镜下行内耳组织学观察.结果表明12个月龄豚鼠内耳组织学呈现自然衰老典型改变,且ABR、MDA、SOD各指标均与老年各指标的改变相一致;3个月龄人工促衰老组无论在内耳组织学的改变及ABR、MDA、SOD与12个月龄豚鼠相比亦基本一致.表明采用豚鼠制作模型以研究老年性聋是可行的.
The guinea pigs of different age groups were randomly divided into two groups: group A and group A were divided into A_1, A_2, A_3 (collectively referred to as natural aging group) at age 3, 6 and 12 months of age; Guinea pigs aged 3 months (hereinafter referred to as artificial aging group) were injected with pro-aging agents for 32 days after being retrobulbar.The rats in each group were sacrificed after ABR and their heart and malondialdehydes , MDA and Superoxide dismutase (SOD) activity in the inner ear were observed under the scanning electron microscope.The results showed that the histology of the inner ear of 12-month-old guinea pig showed the typical changes of aging and the indexes of ABR, MDA and SOD Both of them were consistent with the change of each index in the elderly.The 3-month-old artificial aging group was basically the same as the histological changes in the inner ear and the ABR, MDA and SOD in 12-month-old guinea pigs, indicating that guinea pig model was used to study Senile deafness is feasible.