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目的通过观察慢性铝暴露后大鼠学习记忆行为、肝脏、肾脏、和大脑皮层ERK 1/2含量变化,探讨铝暴露对机体毒性的产生机制,并为治疗铝中毒提供实验依据。方法大鼠自由饮用不同剂量AlCl3水溶液(0.2%、0.4%和0.6%)3个月,实行慢性铝暴露,蛋白免疫印迹(Westem-Blot)方法测定各组细胞外信号调节激酶(ERK 1/2)活力及含量变化。结果与对照组相比较,各个铝暴露组ERK 1/2表达比较,差异有统计学意义(P<0.05)。结论铝暴露可对大鼠身体内重要脏器组织中关键蛋白ERK 1/2产生影响,这可能是铝霉性产生的机制之一。
OBJECTIVE: To observe the changes of learning and memory behaviors, the contents of ERK 1/2 in liver, kidney and cerebral cortex of rats after chronic aluminum exposure, and to explore the mechanism of aluminum exposure on the organism toxicity and to provide experimental evidence for the treatment of aluminum poisoning. Methods Rats were exposed to aqueous AlCl 3 (0.2%, 0.4% and 0.6%) for 3 months. Chronic aluminum exposure and Western blotting were used to determine the expression of extracellular signal-regulated kinase (ERK 1/2 ) Vitality and changes in content. Results Compared with the control group, the expression of ERK 1/2 in each aluminum exposure group was significantly different (P <0.05). Conclusion Aluminum exposure may affect ERK 1/2, a key protein in the tissues of rats, which may be one of the mechanisms responsible for the development of aluminum mold.