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为准确检测水稻白叶枯病菌、细菌性条斑病菌及这两种病菌的复合发生,利用软件DNAStar分析比较这两种菌的部分核酸序列,设计了检测这两种病菌的特异性引物。引物Xoo F-Xoo R能特异性扩增出水稻白叶枯病菌中一条大小162 bp的条带;引物Xooc F1-Xooc R1和Xooc F2-Xooc R2能够分别特异性扩增出水稻细菌性条斑病菌中690 bp和945 bp的条带。通过优化PCR反应条件,成功建立了多重PCR技术,可以对不同国家的水稻白叶枯病菌和细菌性条斑病菌进行准确检测,对由这两种病菌引起的复合侵染实现了准确诊断。
In order to accurately detect the combination of bacterial blight and bacterial leaf spot bacteria and the two pathogens, DNAStar was used to analyze and compare the partial nucleic acid sequences of the two kinds of bacteria, and the specific primers were designed to detect the two pathogens. The Xoo F-Xoo R primer could specifically amplify a 162 bp band in Xanthomonas oryzae, and the primers Xooc F1-Xooc R1 and Xooc F2-Xooc R2 could specifically amplify the rice bacterial streak Bacteria in the 690 bp and 945 bp bands. By optimizing the PCR reaction conditions, the multiplex PCR technique was successfully established to accurately detect bacterial leaf blight and bacterial leaf spot bacteria in different countries, and to accurately diagnose the complex infection caused by these two pathogens.