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使用PCR对 9份采集于 1996年中旬的四川省经血液途径感染的HIV - 1阳性感染者外周血单个核细胞 (PBMCs)样品进行扩增 ,获得HIV - 1膜蛋白 (env)基因的核酸片段 ,并对其C2-V3及邻区 350～ 4 50个核苷酸序列进行了测定和分析。结果表明 ,9份样品中存在B和C两种亚型的HIV - 1毒株序列 ,其中B亚型 5份 ,彼此间的基因离散率为 1.4 % ;C亚型 4份 ,在不包括sc3号样品的情况下彼此间的基因离散率为 2 .6% ,而sc3号样品与其它 3份样品在核苷酸序列上的差异却达 11.5%。与A～E参考亚型及部分B和C亚型代表株序列相比较 ,属四川B亚型的 5个毒株与包括泰国、缅甸及中国云南德宏在内的B亚型毒株序列十分接近 ,基因离散率在 3.6%～4 .3%的范围内 ;属四川C亚型的 4个毒株除sc3外则与代表印度毒株的C亚型共享序列及德宏C亚型毒株序列十分相似 ,其基因离散率均为 3.1% ,与非洲C亚型毒株nof的基因离散率为13.1% ,而sc3号序列与印度、中国德宏和非洲C亚型HIV - 1的基因离散率却在 10 .1%～ 17.2 %的范围内。提示 ,HIV - 1在四川的流行时间不长 ,且其B和除sc3之外的C亚型毒株的传入与流行在云南德宏州的相同亚型HIV - 1毒株密切相关 ,而由sc3所代表的C亚型毒株则存在目前还尚不了解的其它传入来源。
Nine samples of HIV - 1 positive peripheral blood mononuclear cells (PBMCs) from 9 menstrual bloodstream in Sichuan Province collected in mid - 1996 were amplified by PCR, and the nucleic acid fragment of HIV - 1 membrane protein (env) gene was obtained. , And 350-450 nucleotide sequences of C2-V3 and its neighboring regions were determined and analyzed. The results showed that there were 9 strains of HIV - 1 strains of B and C subtypes, among which 5 strains were subtype B, the genetic divergence rate was 1.4%, and 4 subtypes of C were not included sc3 , The gene dispersal rate was 2.6% with respect to each other, whereas the nucleotide sequence difference between sc3 and other 3 samples was 11.5%. Compared with the A ~ E reference subtypes and the partial subtype B and C subtypes, the sequences of 5 strains belonging to Sichuan B subtype and the B subtype including Thailand, Myanmar and China Dehong Close to the gene dispersion rate in the range of 3.6% ~ 4.3%; four strains belonging to the C subtype of Sichuan in addition to the sc3 outside and on behalf of the Indian subtype C subtype and Dehong C subtype strains The sequence divergence was 3.1%, the gene divergence rate was 13.1% with the nof subtype C of Africa, and the gene divergence rate of sc3 with that of India, China Dehong and Africa C subtype HIV - 1 But in the range of 10.1% ~ 17.2%. These results suggest that the prevalence of HIV - 1 in Sichuan Province is not long, and its B and C subtype strains other than sc3 are closely related to the HIV - 1 strain of the same subtype prevailing in Dehong Prefecture, Yunnan Province. However, C subtypes represented by sc3 are present with other sources not yet known.