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快速斑点免疫结合试验（ＤｏｔＩｍｍｕｎｏｂｉｎｄｉｎｇＡｓａｙＤＩＢＡ）是８０年代中期发展起来的固相标记免疫测定技术，其原理是以微孔滤膜为载体，通过渗滤或毛细管作用，使抗原抗体快速进行反应检测数分钟完成，阳性结果在膜上出现着色斑点。近年来，利用微孔滤膜的过滤性能或毛细管作用，创造了各种简便快速的ＤＩＢＡ，如斑点酶免疫渗滤试验，斑点金免疫渗滤试验，斑点免疫层析试验，本文对其原理、反应模式、质量控制、发展趋势及应用作一简要综述
Rapid Dot Immunobinding Assay (DotImmunobindingAsayDIBA) is the development of the mid-80s solid-phase labeling immunoassay technology, the principle is based on microporous membrane as the carrier, by diafiltration or capillary action, the antigen-antibody rapid reaction detected a few minutes to complete Positive results appear on the membrane stain spots. In recent years, the use of microporous membrane filtration performance or capillary action to create a variety of simple and rapid DIBA, such as the stain enzyme immune infiltration test, Auger dot blot test, immunochromatography, the paper on its principles, Reaction mode, quality control, development trend and application as a brief review