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对产氢光合细菌葡萄糖跨膜传输速率、葡萄糖酶酵解速率进行了实验研究,对胞内葡萄糖浓度、葡萄糖传输渗透系数进行了计算.实验结果表明,胞外初始底物浓度为50～100mmol/L时,葡萄糖传输速率、胞内葡萄糖浓度总体都随胞外初始底物浓度的增大而增大,但胞外初始底物浓度增大至150mmol/L时,对葡萄糖跨膜传输产生了限制作用,葡萄糖传输速率和胞内葡萄糖浓度均相应减小;计算得到胞内葡萄糖浓度最小值为0.34mmol/L,最大为5.87mmol/L;初始底物浓度为15～35mmol/L时,细胞破碎液葡萄糖酶酵解速率随底物浓度的增大而增大;胞外初始底物浓度为50～150mmol/L时,葡萄糖代谢速率受葡萄糖传输速率控制,产氢光合细菌葡萄糖平均渗透系数为231.13cm3h-1g(DW)-1,葡萄糖传输速率主要受胞内外葡萄糖浓度势差控制;75mmol/L为较有利于葡萄糖跨膜传输的最佳胞外初始底物浓度.图5参11
The transmembrane transport rate of glucose and the rate of glucosease digestion of hydrogen-producing photosynthetic bacteria were experimentally studied, and the intracellular glucose concentration and the glucose permeation coefficient were calculated.The experimental results showed that the initial concentration of extracellular substrate was 50 ~ 100mmol / L, the glucose transport rate and the intracellular glucose concentration all increased with the increase of the initial extracellular substrate concentration. However, when the extracellular initial substrate concentration was increased to 150 mmol / L, the glucose transmembrane transport was limited The glucose transport rate and intracellular glucose concentration decreased correspondingly. The minimum intracellular glucose concentration was 0.34mmol / L and the maximum was 5.87mmol / L. When the initial substrate concentration was 15 ~ 35mmol / L, the cells were broken When the concentration of extracellular initial substrate was 50 ~ 150mmol / L, the rate of glucose metabolism was controlled by the glucose transport rate, and the average glucose permeability coefficient of hydrogen-producing photosynthetic bacteria was 231.13 cm3h-1g (DW) -1. The glucose transport rate was mainly controlled by the intracellular and extracellular glucose concentration difference; 75mmol / L was the best extracellular initial substrate concentration for transmembrane transport of glucose.