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本试验利用外源DNA导入技术,研究了大麦DNA导入小麦品种后,D_1,D_2和D_3代的抗白粉病变异株(系)在不同条件下的抗性表现及产量构成、籽粒蛋白质和氨基酸含量的变化。试验结果表明,导入外源DNA的D_1代小麦檀株出。现了抗白粉病等多种变异类型,其中免疫和高抗白粉病变异株古2.77%,且抗性能够向子代传递,其D_2代在大田自然发病和温室接菌条件下,有5个株系抗性保持稳定,8个株系有分离,其中一个株系在D_2和D_3代抗性均稳定。在田间D_2代有2个稳定株系(D_2-20,D_2-29)的籽粒粗蛋白质含量,比受体分别高20.3%和15.76%,17种氨基酸总量分别高23.4%和27.5%。在温室这些性,状的数值也明显高于受体。
In this study, the resistance of powdery mildew resistant lines D_1, D_2, and D_3 to barley powdery mildew resistant to different conditions was studied by using exogenous DNA introduction technology. The results showed that the resistance and yield components of grain protein and amino acid The change. The results showed that the introduction of foreign DNA D_1 generation of wheat Tannong out. A variety of resistant varieties such as powdery mildew were found, of which 2.77% were immune and highly resistant to powdery mildew, and the resistance was able to be passed on to offspring. The D_2 generation had 5 natural onset and greenhouse inoculation conditions Strain resistance remained stable, with eight isolates, one of which was stable on the D_2 and D_3 generations. The crude protein content of two stable lines (D_2-20, D_2-29) in field D_2 was 20.3% and 15.76% higher than that of the recipient, respectively. The total amount of 17 amino acids was 23.4% and 27.5% higher than that of the recipient respectively. In the greenhouse these sexual, the shape of the value was significantly higher than the receptor.