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一种监测氯霉素血药浓度的高效液相色谱法,分析条件如下:分析柱:μbondpakC18;流动相:甲醇:1％乙酸(45:55);流速:1.0ml/min;检测波长:UV285nm,本方法样品峰分离好,保留时间为7.2min。峰高与浓度(1～50mg/L)呈线性(r=0.9999);血清提取回收率高,平均为92±3％;重复性好,变异系数<2.4％;正确性高,9个血清样品的测得浓度与配制浓度的差异为3.7±2.9％,特异性高,17种抗菌药物对氯霉素样品峰无干扰。本方法简单、灵敏、可靠,完全能满足临床对氯霉素血液及其他体液浓度的监测。
A HPLC method for monitoring the concentration of chloramphenicol in blood was as follows: Analytical column: μbondpak C18; Mobile phase: Methanol: 1% Acetic acid (45:55); Flow rate: 1.0 ml / min; Detection wavelength: UV285 nm , The method of sample peak separation, retention time was 7.2min. The peak height was linear with the concentration (1 ~ 50mg / L) (r = 0.9999), the recovery rate of serum was high with an average of 92 ± 3%, the repeatability was good, the coefficient of variation was <2.4% Of the measured concentration and the concentration of the difference was 3.7 ± 2.9%, high specificity, 17 kinds of antibacterial drugs chloramphenicol sample peak without interference. The method is simple, sensitive and reliable, and can fully meet the clinical monitoring of chloramphenicol blood and other body fluid concentrations.