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应用免疫组织化学技术检测29例肝细胞癌（肝癌）和23例肝硬化标本中突变型p53蛋白的表达，并探讨后者与肝癌临床病理学特征之间的关系。结果：肝癌突变型p53蛋白表达阳性率为70％（20／29），癌旁组织阳性率为60％（18／29），肝硬化组织阳性率为30％（7／23），有血管侵犯的12例肝癌变变型p53蛋白表达均为阳性，而无血管侵犯的17例中仅8例为阳性（P＜0．05）；突变型p53蛋白阳性组血清甲胎蛋白（AFP）明显高于阴性组（8370±4764ng／ml比98．7±64．3ng／ml，P＜0．05）。提示：P53蛋白表达在肝硬化时即发生了异常；p53蛋白异常可能是AFP基因被激活的原因之一；p53蛋白异常有利于肝癌向门静脉转移。
Immunohistochemistry was used to detect the expression of mutant p53 protein in 29 cases of hepatocellular carcinoma (HCC) and 23 cases of cirrhosis, and to explore the relationship between the latter and the clinicopathological features of HCC. Results: The positive rate of p53 protein expression in liver cancer was 70% (20/29). The positive rate in adjacent tissues was 60% (18/29). The positive rate in liver cirrhosis was 30% (7/23). The positive expression of p53 protein was positive in 12 cases of hepatocellular carcinoma, and only 8 cases were positive in 8 cases without vascular invasion (P<0.05). The level of serum alpha-fetoprotein (AFP) was significantly higher in the mutant p53 protein positive group than in 8 cases. The negative group (8370±4764 ng/ml vs 98.7±64.3 ng/ml, P<0.05). It is suggested that abnormal expression of P53 protein occurs in liver cirrhosis. Abnormality of p53 protein may be one of the reasons that AFP gene is activated. The abnormality of p53 protein is conducive to liver cancer metastasis to portal vein.