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OBJECTIVE:To investigate the effect of Chaiqin Chengqi decoction (CQCQD) on acute pancreatitis (AP) by janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in vitro and in vivo.METHODS:AP was induced by caerulein both in AR42J cells and in mice.AR42J cells were divided into five groups:the control group,the AP group,the CQCQD group,JAK/STAT signaling pathway inhibitor AG490 group,and the CQCQD and AG490 group.After induction,cellular supatant of five groups were collected for measuring the concentrations of inflammatory cytokine amylase,interleukin 6 (IL-6),tumor necrosis factor α (TNF-α),interleukin 1β (IL-1β),nuclear factor κB (NF-κB) by enzyme-linked immunosorbent assay and the expression of JAK-2,STAT-3 signaling transduction proteins by West blot,respectively.Experiments in mice were performed similar to that of in AR42J cells.RESULTS:Treatment of AR42J cells with CQCQD reduced the pancreatic injury and negatively regulated the activities of amylase,as well as inhibited expression of several inflammatory cytokines such as IL-6,TNF-α,IL-1β,NF-κB.Administration of CQCQD significantly inhibited JAK-2 activation and down-regulated phosphorylation of downstream substrate STAT-3 the same as AG490,resulting in inhibition of inflammatory mediators and amelioration of pancreatitis.CONCLUSION:The results suggested that CQCQD exerted anti-inflammatory effects on AP via reducing expression and phosphorylation of JAK and STAT.