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单纯形法优化色谱条件,具有道理简单、操作方便,有效等特点。本文在 M-201液品填充柱上,以色谱响应函数 CRFRT 作为谱色分离质量的评价标准,应用单纯形法优化二甲苯异构体的色谱分离条件,确定了最佳操作条件。以柱温73℃、载气流量38ml/min 的最优条件分析二甲苯异构体,既可保证获得良好的分离效果,又可大大缩短分析时间,适于实际应用。
Simplex method to optimize chromatographic conditions, with the reason simple, easy to operate, effective and so on. On the M-201 liquid packed column, the chromatographic response function CRFRT was used as the evaluation standard for spectral color separation. The chromatographic conditions for the separation of xylene isomers were optimized by simplex method, and the optimum operating conditions were determined. Analysis of xylene isomers at a column temperature of 73 ° C and a carrier gas flow rate of 38 ml / min can ensure a good separation effect, greatly shorten the analysis time and be suitable for practical application.