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[Objective] To extract polysaccharides in Abutilon indicum( Linn.) Sweet using cellulase,determine the optimal extraction process through orthogonal design,and treat the extracted polysaccharides and study the anti-oxidant activity. [Methods]Single factor experiment and orthogonal experiment were carried out to study effects of p H value of buffer solution,enzyme volume,enzymolysis temperature,and extraction time on extraction of polysaccharides in Abutilon indicum( Linn.) Sweet. Polysaccharides in Abutilon indicum( Linn.) Sweet was prepared and purified,DPPH and hydroxyl free radicals scavenging effect and Fe3 +reducing ability were measured,and anti-oxidant ability was evaluated according to experimental results. [Results]The conditions for optimal extraction process: p H 6. 5,enzyme volume 5 m L,enzymolysis temperature 50℃,extraction time 1. 5 h,average extraction rate 5. 223%,and RSD 1. 68%. Anti-oxidant experiment indicates that polysaccharides in Abutilon indicum( Linn.) Sweet has significant effect on scavenging of free radicals. [Conclusion]In certain range of concentration,there is dose-response relationship between polysaccharides in Abutilon indicum( Linn.) Sweet and scavenging rate of free radicals and the scavenging activity is significant. This study is expected to provide a certain scientific basis for comprehensive use and development of Abutilon indicum( Linn.) Sweet.
[Objective] To extract polysaccharides in Abutilon indicum (Linn.) Sweet using cellulase, determine the optimal extraction process through orthogonal design, and treat the extracted polysaccharides and study the anti-oxidant activity. [Methods] Single factor experiment and orthogonal experiment were carried out to study effects of pH value of buffer solution, enzyme volume, enzymolysis temperature, and extraction time on extraction of polysaccharides in Abutilon indicum (Linn.) Sweet. Polysaccharides in Abutilon indicum (Linn.) Sweet was prepared and purified, DPPH and hydroxyl free radicals scavenging effect and Fe3 + reducing ability were measured, and anti-oxidant ability was evaluated according to experimental results. [Results] The conditions for optimal extraction process: p H 6.5, enzyme volume 5 m L, enzymolysis temperature 50 ℃, extraction time 1. 5 h, average extraction rate 5. 223%, and RSD 1. 68%. Anti-oxidant experiment indicates that polysaccharides in Abutilon indicum (Linn.) Sweet There is a significant effect on scavenging of free radicals. [Conclusion] In certain range of concentration, there is dose-response relationship between polysaccharides in Abutilon indicum (Linn.) Sweet and scavenging rate of free radicals and the scavenging activity is significant. This study is expected to provide a certain scientific basis for comprehensive use and development of Abutilon indicum (Linn.) Sweet.