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作者研究了无机汞和有机汞对体外骨髓及肿瘤细胞的毒性,并用硒逆转其变化。方法:实验动物选用BDF_1和DBA/2雌性小鼠,鼠龄8～12周。所有试剂均用生理盐水配制,使用前用0.45μm筛过滤消毒。取骨髓和P815株细胞,分别置于35毫米组织培养皿中培养(此法1977年Uyeki氏已作介绍)。骨髓细胞培养至1×10~5个有核细胞/毫升,P815株细胞至1×10~3个细胞/毫升,这两类细胞株数大约每个培养皿为200个。株数按对照组、汞实验组和汞+亚硒酸盐组分别计数。结果以实验组株数与对照组株数比率表示。第一个实验,将亚硒酸盐和汞先加至培养基中,混合后用琼脂固定,接种细胞后培养一周。第
The authors studied the toxicity of inorganic and organic mercury to bone marrow and tumor cells in vitro and reversed the changes with selenium. Methods: BDF_1 and DBA / 2 female mice were selected for experimental animals, aged 8-12 weeks. All reagents were prepared with saline, filtered before use 0.45μm screen disinfection. Bone marrow and P815 cells were harvested and cultured in 35 mm tissue culture dishes (this method was introduced in 1977 by Uyeki). Bone marrow cells are cultured to 1 × 10 5 nucleated cells / ml, P815 cells to 1 × 10 3 cells / ml, and the number of these two types of cells is about 200 per culture dish. The number of strains was counted by control group, mercury experimental group and mercury + selenite group respectively. Results were expressed as the number of strains in the experimental group and the control group. In the first experiment, selenite and mercury were first added to the medium, mixed and then fixed with agar. The cells were inoculated and cultured for one week. No.