论文部分内容阅读
ＢＡＬＢ／ｃ小鼠荷腹水型肝癌，连续灌喂中药伍铜铁复方制剂（ＣＨＣＦ）１０天，流式细胞光度分析结果表明：ＣＨＣＦ可使癌细胞ＤＮＡ含量降低；增殖指数（ＰＩ）下降（对照组８３．４±２．６，给药组７８．８±１．５或对照组６７．２±１．３，给药组６４．２±１．６，Ｐ＜０．０５）；Ｇ１期细胞数明显增多，而Ｓ＋Ｇ２Ｍ期细胞数明显减少（Ｐ＜０．０５～０．０１）；ＤＮＡ组方图中二倍体峰明显增高变大，而多倍体峰变小。再移植实验证明：经ＣＨＣＦ处理过的癌细胞再移植的动物有２／１０不长肿瘤，肿瘤抑制率为７１．７％～８８．３％；再移植动物存活期明显延长，从２６．１±１１．８天延长至３８．１±９．６天或延长至３９．６±７．２天，生命延长率（Ｔ／Ｃ）分别为４６％和５２％。因而证明：该制剂降低了小鼠肝癌细胞的恶性程度，其作用机理可能是激活癌细胞膜的氧化磷酸化过程。
BALB/c mice were treated with ascites-type liver cancer and were continuously given Chinese herbal compound (CHCF) for 10 days. Flow cytometric analysis showed that CHCF could reduce the DNA content of the cancer cells and the proliferation index (PI) decreased. 83.4±2.6 in group, 78.8±1.5 in control group or 67.2±1.3 in control group, 64.2±1.6 in administration group (P<0.05); G1 phase The number of cells increased significantly, while the number of cells in the S+G2M phase decreased significantly (P<0.05-0.01); the diploid peaks in the DNA group map increased significantly and became larger, while the polyploid peaks became smaller. Re-transplantation experiments showed that 20% of tumors re-transplanted by CHCF-treated cancer had no tumors, and the tumor inhibition rate was 71.7% to 88.3%; the survival of re-transplanted animals was significantly longer, from 26.1. The ±11.8 days extended to 38.1 ± 9.6 days or 39.6 ± 7.2 days, with a life extension rate (T/C) of 46% and 52%, respectively. Therefore, it is proved that the preparation reduces the degree of malignancy of mouse hepatoma cells, and its mechanism of action may be activation of the oxidative phosphorylation of cancer cell membranes.