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目的:研究Smoothened(SMO)在肝癌和肝硬化中的表达及临床意义。方法:选取组织标本后,运用石蜡包埋,切片后,HE染色,构建组织芯片,免疫组织化学和原位杂交方法检测Smo蛋白在肝癌和肝硬化中的表达。结果:Smo蛋白在肝癌细胞浆、良性肝肿瘤组织细胞浆、肝硬化组织中强染色,在正常组织中无染色。并且在典型肝硬化中强染色,在中度肝硬化中弱阳性。结论:Smo蛋白表达与肝癌的发生有关,Smo基因的高表达可能激活某种机制而参与诱导肝癌的发生。可能是通过异常激活Sonic hedgchog信号通路,从而诱导肝癌的发生与发展。
Objective: To study the expression and clinical significance of Smoothened (SMO) in hepatocellular carcinoma and cirrhosis. Methods: Tissue samples were selected and embedded in paraffin. The sections were stained with HE, stained with HE staining, and then the expression of Smo protein was detected by immunohistochemistry and in situ hybridization. RESULTS: The Smo protein was strongly stained in the cytoplasm of hepatocellular carcinoma, cytoplasm of benign hepatic tumor and cirrhosis, but not in normal tissues. Strongly stained in typical cirrhosis and weakly positive in moderate cirrhosis. Conclusion: The expression of Smo protein is related to the occurrence of hepatocellular carcinoma. The high expression of Smo gene may activate certain mechanism and participate in the induction of hepatocellular carcinoma. May be through abnormal activation of Sonic hedgchog signaling pathway, thereby inducing the occurrence and development of liver cancer.