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精子必须经过获能和顶体反应(AR)才具受精能力。利用获能和AR 前后精子细胞内和精子表面大分子的变化,可探索新的避孕办法和男性不育诊断及治疗的新途径。本文将正常人精子于体外在BWW-BSA 培养基中获能,用钙离子载体A 23187诱导人精子进行AR(以三重染色和金霉素荧光染色两种方法检测这些精子的AR 率为50%左右),然后用这些新鲜的人精子作为免疫原,制备了23个抗人精子单克隆抗体,其中21个为IgM,2个分别为IgG_1和IgG_2。根据23个单克隆抗体与经获能和AR 处理的精子以及未处理精子的不同免疫反应,将它们分为A(AR 精子反应组)、B(未处理精子反应组)和C(双重精子反应组)三组,并测定了这些单克隆抗体与人的某些白血病细胞系的交叉反应。
Sperm must be capacitated and acrosomelyzed (AR) to be fertilized. Using the changes of capacitation and macromolecules in spermatids and sperms before and after AR, new methods of contraception and diagnosis and treatment of male infertility can be explored. In this study, normal human spermatozoa were induced in vitro in BWW-BSA medium and human spermatozoa AR were induced by calcium ionophore A 23187. The AR rate of these sperm was 50% by triple staining and chlortetracycline staining, ), And then use these fresh human sperm as immunogen, prepared 23 anti-human sperm monoclonal antibodies, of which 21 were IgM, two were IgG_1 and IgG_2. They were divided into A (AR sperm response group), B (untreated sperm response group) and C (double sperm response) based on the different immune responses of 23 monoclonal antibodies to capacitated and AR-treated sperm as well as to untreated sperm Group) and determined the cross-reactivity of these monoclonal antibodies with certain human leukemia cell lines.